ArticleTranslational perioperative and pain medicine2021
The Microbial Flora in an Experimental Polymicrobial Abdominal Sepsis Model Probed by 16S rRNA Sequencing.
Article in Translational perioperative and pain medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Optimization of a fresh fecal intraperitoneal injection sepsis model and its divergent dynamics from cecal ligation and puncture in mice.Laboratory animal research · 2026Article
- BRD4 modulates antimicrobial defense via non-canonical NRF2 activation in macrophages to confer protection against sepsis.PLoS pathogens · 2026Article
- Therapeutic targeting of endothelial calcium signaling accelerates the resolution of lung injury.Signal transduction and targeted therapy · 2025Article
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Authors and funding
4 authors.
Funding
Abstract
backgroundCecal ligation and puncture (CLP) surgery is a widely used preclinical model to induce and study sepsis because it is considered to recapitulate the course of human sepsis the most. This model is highly dependent on the polymicrobial gut flora and represents polymicrobial abdominal sepsis. While the majority of studies using CLP model have focused on the delineation of host immune responses, a limited number of reports have described the composition of microbial strains in this model, although microbial composition can significantly affect the outcome of sepsis in general.
methodsCLP surgery was performed in mice on C57BL6/J from the Jackson laboratory. We examined the composition of microbes at the peritoneal cavity using 16S rRNA sequencing after CLP surgery at 12 and 24 hours. Baseline cecal microbial flora was also analyzed.
resultsThe bacteria strains from the initial cecum flora consisted of mixed aerobic and anaerobic flora. There was a significant change of bacteria flora from the peritoneal cavity between 12 and 24 hours following CLP surgery. Particularly a significantly increased proportion of anaerobic microbes were noted at 24 hours after CLP surgery. We also tested bacterial composition of cecal flora of mice on the same background from the same vendor 6 months later. Baseline cecal microbial flora was different from earlier mice, showing that baseline cecal flora could be different depending on the batch of mice.
conclusionThere was a dynamical chance of peritoneal microbes during CLP sepsis. Potential difference in baseline cecal flora should be kept in mind upon CLP surgery even when using mice from the same vendor.
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33521166PMC7840154What OpenQuestion holds
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