Evidence map›Paper›PMID 33520960›Full record

ArticleFrontiers in bioengineering and biotechnology2020

A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125

Wenting Xu, Yanxiu Mo, Yu He, Yunpeng Fan, Guomin He, Wen Fu, Shujuan Chen, Jinhui Liu, Wenbin Liu, Liangyue Peng and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Wenting XuState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Yanxiu MoState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Yu HeState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Yunpeng FanState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Guomin HeState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Wen FuState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Shujuan ChenState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Jinhui LiuState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Wenbin LiuState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Liangyue PengState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Yamei XiaoState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
Hunan Normal University · CNXiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is suggested that enhancement of the p53 signaling pathway and weakening of the spindle assembly checkpoint are associated with the SP600125-induced cell cycle arrest. Using preliminary SP600125 treatment, the samples of the cultured fish cells and the fish tissues display a great number of chromosome splitting phases. Summarily, SP600125 can provide a new protocol of chromosomes preparation for karyotype analysis owing to its interference with prometaphase of mitosis.

Indexed as

chromosomeendomitosisp53SP600125spindle assembly checkpoint

Identifiers

PMID33520960
PMCPMC7838586
OpenAlexW3118529605

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.