Evidence map›Paper›PMID 33520948›Full record

ArticleFrontiers in bioengineering and biotechnology2020

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement.

Wayne M Barnes, Zhian Zhang, Milko B Kermekchiev

Open access · goldAbstract read
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.Critical reviews in biochemistry and molecular biology · 2026
    Review
  2. Mechanism and application of Taq DNA polymerase in TaqMan qPCR.Frontiers in bioengineering and biotechnology · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Engineering psychrophilic polymerase for nanopore long-read sequencing.Frontiers in bioengineering and biotechnology · 2024
    Article
  7. Article
  8. Article
  9. Bst polymerase - a humble relative of Taq polymerase.Computational and structural biotechnology journal · 2023
    Review
  10. Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022
    Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Wayne M BarnesDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States.
Zhian ZhangDNA Polymerase Technology, Inc., St. Louis, MO, United States.
Milko B KermekchievDNA Polymerase Technology, Inc., St. Louis, MO, United States.
DNA Polymerase Technology (United States) · US

Funding

Direct RT-PCR detection of RNA pathogens in crude samplesR44GM088948 · NIGMS · DNA POLYMERASE TECHNOLOGY, INC. · PI ZHANG, ZHIAN · 2014 to 2015
$899k
FLUORESCENT AMINO ACID PROBE OF TEMPLATE-STRAND BASESR21HG006291 · NHGRI · WASHINGTON UNIVERSITY · PI BARNES, WAYNE MORRIS · 2011 to 2012
$608k
NHGRI NIH HHS R21 HG006291NIGMS NIH HHS R44 GM088948
6 · The paper itself

Abstract

A change of an aspartic acid to asparagine of Taq (

Indexed as

diagnosticspolymerase chain reaction with reverse transcriptionRNA detectionRT-LAMPRT-PCRTaq DNA polymerase

Identifiers

PMID33520948
PMCPMC7841393
OpenAlexW3119863989

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.