ArticleBiomedical optics express2021
Hyperspectral imaging and spectral unmixing for improving whole-body fluorescence cryo-imaging.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
- Evaluation of a near-infrared version of TMR-PEG1k, a high-performance untargeted contrast agent for fluorescence-guided surgery, using fluorescence cryotomography.Journal of biomedical optics · 2025Article
- Dynamic oxygen assessment techniques enable determination of anesthesia's impact on tissue.Scientific reports · 2025Article
- GFP-on mouse model for interrogation of in vivo gene editing.Nature communications · 2025Article
- Elucidating the effect of tumor and background region-of-interest selection on the performance metrics used to assess fluorescence imaging.Journal of biomedical optics · 2025Article
- Comparing spatial distributions of ALA-PpIX and indocyanine green in a whole pig brain glioma model using 3D fluorescence cryotomography.Journal of biomedical optics · 2025Article
- Two-color diffuseJournal of biomedical optics · 2024Article
- Whole-body fluorescence cryotomography identifies a fast-acting, high-contrast, durable contrast agent for fluorescence-guided surgery.Theranostics · 2024Article
- SpeCamX: mobile app that turns unmodified smartphones into multispectral imagers.Biomedical optics express · 2023Article
- Current and Future Applications of Fluorescence Guidance in Orthopaedic Surgery.Molecular imaging and biology · 2023Review
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
- Denoising multiplexed microscopy images in n-dimensional spectral space.Biomedical optics express · 2022Article
- Automated multicolor mesoscopic imaging for the 3-dimensional reconstruction of fluorescent biomarker distribution in large tissue specimens.Biomedical optics express · 2022Article
- Trends in hyperspectral imaging: from environmental and health sensing to structure-property and nano-bio interaction studies.Analytical and bioanalytical chemistry · 2022Article
- A hyperspectral approach for recovering agent excretion biodistributions using whole-body fluorescence cryo-imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2021Article
- A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2021Article
- Comparing fluorescent contrast agents for fluorescence guided surgery using 3-D cryo-imaging.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- A new candidate agent for fluorescence guided neurosurgery produces high, persistent tumor contrast shortly after administration.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- Whole-brain MR-registered cryo-imaging of a porcine-human glioma model to compare contrast agent biodistributions.Proceedings of SPIE--the International Society for Optical EngineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Whole-animal fluorescence cryo-imaging is an established technique that enables visualization of the biodistribution of labeled drugs, contrast agents, functional reporters and cells in detail. However, many tissues produce endogenous autofluorescence, which can confound interpretation of the cryo-imaging volumes. We describe a multi-channel, hyperspectral cryo-imaging system that acquires densely-sampled spectra at each pixel in the 3-dimensional stack. This information enables the use of spectral unmixing to isolate the fluorophore-of-interest from autofluorescence and/or other fluorescent reporters. In phantoms and a glioma xenograft model, we show that the approach improves detection limits, increases tumor contrast, and can dramatically alter image interpretation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.