ArticleBiomedical optics express2021
Adaptive optics enables aberration-free single-objective remote focusing for two-photon fluorescence microscopy.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Review
- Review
- Adaptive aberration correction using an electrowetting array.Applied physics letters · 2023Article
- Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping.Nature biotechnology · 2022Article
- Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror.JPhys photonics · 2021Article
- Robust functional imaging of taste sensation with a Bessel beam.Biomedical optics express · 2021Article
- Precompensation of 3D field distortions in remote focus two-photon microscopy.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Two-photon fluorescence microscopy has been widely applied to three-dimensional (3D) imaging of complex samples. Remote focusing by controlling the divergence of excitation light is a common approach to scanning the focus axially. However, microscope objectives induce distortion to the wavefront of non-collimated excitation beams, leading to degraded imaging quality away from the natural focal plane. In this paper, using a liquid-crystal spatial light modulator to control the divergence of the excitation beam through a single objective, we systematically characterized the aberrations introduced by divergence control through microscope objectives of NA 0.45, 0.8, and 1.05. We used adaptive optics to correct the divergence-induced-aberrations and maintain diffraction-limited focal quality over up to 800-µm axial range. We further demonstrated aberration-free remote focusing for
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Registered trials
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