ArticlePoultry science2021
Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production.
Article in Poultry science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Multicomponent mineral-phytogenic supplementation reduces Ascaridia galli shedding and systemic mycotoxin biomarkers while improving health and productive performance in laying hens.Veterinary world · 2026Article
- Dual-strain Bacillus subtilis 597 and 600 complex improves productive performance and nutrient digestibility, egg quality and physiological status in early-to-peak phase laying hens.Poultry science · 2026Article
- Effects of dietary PQQ·NaPoultry science · 2026Article
- Encapsulated Bacillus sp. THPS1, a novel thermophilic probiotic, enhances productivity and metabolic health in laying hens.Poultry science · 2026Article
- Effects of ProbioticAnimals : an open access journal from MDPI · 2026Article
- Optimizing eggshell quality in extended laying periods: Insights and strategies.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Probiotic-enzyme complex improves laying performance in laying hens fed low-protein diets by modulating gut microbiota and host metabolism.Frontiers in veterinary science · 2026Article
- Compound Probiotics Enhance Performance, Antioxidant Status, and Cecal SCFAs in Post-Molting Broiler Breeders.Animals : an open access journal from MDPI · 2025Article
- Disequilibrium feeding pattern more consistent with egg-laying physiology improves eggshell quality of laying hens during late laying period.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Dietary glycyrrhizin enhances reproductive performance by improving intestinal microbiota, liver lipid metabolism and ovarian senescence in aged breeder hens.Journal of animal science and biotechnology · 2025Article
- Supplementation effects of feed additive containing eggshell,Journal of animal science and technology · 2025Article
- Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens.Animal bioscience · 2025Article
- Article
- Eggshell Quality Traits and Transcriptome Gene Screening Between Yunnong and Jingfen Chicken Breeds.Biology · 2024Article
- Dietary supplementation with calcitriol or quercetin improved eggshell and bone quality by modulating calcium metabolism.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Integration of Whole-Genome Resequencing and Transcriptome Sequencing Reveals Candidate Genes in High Glossiness of Eggshell.Animals : an open access journal from MDPI · 2024Article
- Dietary 25-Hydroxycholecalciferol Supplementation as a Vitamin DAnimals : an open access journal from MDPI · 2024Article
- The flexible linker and CotG were more effective for the spore surface display of keratinase KERQ7.World journal of microbiology & biotechnology · 2023Article
- Genome-Assisted Probiotic Characterization and Application ofMicroorganisms · 2023Article
- Effects ofLife (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal morphology, and relative calbindin-D28k (CALB1) mRNA level of laying hens in the late phase of production. An experiment employing a 2 × 3 factorial arrangement of 3 levels of Ca (3.5, 4.0, and 4.5%) and the absence or presence of B. subtilis was carried out with a total of 576 Hy-Line Brown laying hens aged 72 to 79 wk. Every group had 8 replicates of 12 birds each. The results showed that 4.0 and 4.5% Ca levels improved (P < 0.05) apparent retention and serum Ca content of aged laying hens. Compared with the 3.5% Ca level, the 4.0% Ca level in diets increased (P < 0.05) thickness, eggshell weight, shell ratio, and eggshell Ca content of aged laying hens. Moreover, breaking strength, thickness, eggshell weight, shell ratio, eggshell Ca content, apparent retention of Ca in g/day, apparent retention of Ca in percent, villus height, villus height/crypt depth, serum Ca level, and relative CALB1 mRNA level of aged laying hens were all increased (P < 0.05) by B. subtilis supplementation in diets. The supplemental B. subtilis decreased feed conversion ratio (P = 0.001) significantly. In addition, there was an interaction effect between increased Ca levels from 3.5 to 4.5% and B. subtilis supplementation on crypt depth in the duodenum (P < 0.05). In conclusion, we found that both the increase in dietary Ca level from 3.5 to 4.5% and B. subtilis supplementation could enhance intestinal Ca absorption and improve eggshell quality of laying hens in the late phase of production (72-79 wk of age). Dietary supplementation of B. subtilis accompanying the 4.0% Ca level was appropriate in enhancement of eggshell quality.
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