ArticleBone & joint research2021
Effects of different tissue specimen pretreatment methods on microbial culture results in the diagnosis of periprosthetic joint infection.
Article in Bone & joint research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.
- Optimized use and performance of culture for periprosthetic joint infection diagnosis: a comprehensive literature review.Clinical microbiology reviews · 2025Pooled it
- Is sonication superior to dithiothreitol in diagnosis of periprosthetic joint infections? A meta-analysis.International orthopaedics · 2022Pooled it
- Currently applied sonication protocols for the detection of implant-associated infections in orthopaedics: a scoping review.Journal of clinical microbiology · 2026Article
- Effective therapy for periprosthetic joint infection through sequential application of non-contact low-frequency ultrasonic debridement and antiseptic solutions.Bone & joint research · 2026Article
- Adjunctive intraoperative pulsed direct current with cefazolin: a rabbit proof-of-concept model for prevention of prosthetic joint infection.Frontiers in cellular and infection microbiology · 2026Article
- A Novel Approach for Tissue Analysis in Joint Infections Using the Scattered Light Integrating Collector (SLIC).Biosensors · 2025Article
- Comparison of the efficacy of one-stage revision surgery guided by precise pathogen diagnosis and conventional two-stage revision for chronic knee periprosthetic joint infection.Arthroplasty (London, England) · 2025Article
- A prospective evaluation of the inoculation of homogenised tissue and bone biopsies in blood culture bottles for the diagnosis of orthopaedic-device-related infections.Journal of bone and joint infection · 2025Article
- Evaluation of High-Throughput Gene Chip Array for Enhanced Diagnosis of Bone and Joint Infections: A Comparative Analysis with mNGS and Conventional Culture Methods.Infection and drug resistance · 2025Article
- Systemic antibiotic prophylaxis in arthroplasty - a narrative review of how many doses are optimal.EFORT open reviews · 2024Review
- Can pre-analytical procedures improve microbiological culture yield in patients with periprosthetic infections?BMC microbiology · 2024Article
- Rapid high-throughput processing of tissue samples for microbiological diagnosis of periprosthetic joint infections using bead-beating homogenization.Journal of clinical microbiology · 2024Article
- Bacteria Living in Biofilms in Fluids: Could Chemical Antibiofilm Pretreatment of Culture Represent a Paradigm Shift in Diagnostics?Microorganisms · 2024Review
- Shedding light on negative cultures in osteoarticular infections: leveraging mNGS to unravel risk factors and microbial profiles.Frontiers in cellular and infection microbiology · 2024Article
- Improved cure rate of periprosthetic joint infection through targeted antibiotic therapy based on integrated pathogen diagnosis strategy.Frontiers in cellular and infection microbiology · 2024Article
- Peking University First Hospital Procedure for Culturing Pathogenic Microorganisms for Bone and Joint Infection.Infection and drug resistance · 2024Article
- Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection.Bone & joint research · 2023Article
- Multiplex PCR-based next generation sequencing as a novel, targeted and accurate molecular approach for periprosthetic joint infection diagnosis.Frontiers in microbiology · 2023Article
- Patient-Reported Outcome on Quality of Life and Pain after Revision Arthroplasty for Periprosthetic Joint Infection: A Cross-Sectional Study.Journal of clinical medicine · 2022Article
- Evaluation of two different semi-automated homogenization techniques in microbiological diagnosis of periprosthetic joint infection: disperser vs. bead milling method.BMC infectious diseases · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsMicrobiological culture is a key element in the diagnosis of periprosthetic joint infection (PJI). However, cultures of periprosthetic tissue do not have optimal sensitivity. One of the main reasons for this is that microorganisms are not released from the tissues, either due to biofilm formation or intracellular persistence. This study aimed to optimize tissue pretreatment methods in order to improve detection of microorganisms.
methodsFrom December 2017 to September 2019, patients undergoing revision arthroplasty in a single centre due to PJI and aseptic failure (AF) were included, with demographic data and laboratory test results recorded prospectively. Periprosthetic tissue samples were collected intraoperatively and assigned to tissue-mechanical homogenization (T-MH), tissue-manual milling (T-MM), tissue-dithiothreitol (T-DTT) treatment, tissue-sonication (T-S), and tissue-direct culture (T-D). The yield of the microbial cultures was then analyzed.
resultsA total of 46 patients were enrolled, including 28 patients in the PJI group and 18 patients in the AF group. In the PJI group, 23 cases had positive culture results via T-MH, 22 cases via T-DTT, 20 cases via T-S, 15 cases via T-MM, and 13 cases via T-D. Three cases under ongoing antibiotic treatment remained culture-negative. Five tissue samples provided the optimal yield. Any ongoing antibiotic treatment had a relevant influence on culture sensitivity, except for T-DTT.
conclusionT-MH had the highest sensitivity. Combining T-MH with T-DTT, which requires no special equipment, may effectively improve bacterial detection in PJI. A total of five periprosthetic tissue biopsies should be sampled in revision arthroplasty for optimal detection of PJI. Cite this article:
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.