ArticlePoultry science2021
Transcriptome analysis and identification of age-associated fertility decreased genes in hen uterovaginal junction.
Article in Poultry science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Multi-omics analyses reveal steroid hormone and antioxidant pathways regulating sperm storage capacity in female Muscovy ducks (Cairina moschata).Poultry science · 2026Article
- Genome-wide association study reveals putative genetic determinants of longitudinal immune responses to avian influenza and Newcastle disease in chickens.Genetics, selection, evolution : GSE · 2026Article
- Multi-Omics Reveals Molecular and Genetic Mechanisms Underlying Egg Albumen Quality Decline in Aging Laying Hens.International journal of molecular sciences · 2025Article
- Age-Associated Proteomic Changes in Human Spermatozoa.International journal of molecular sciences · 2025Article
- Comparative transcriptome analysis of bull X- and Y-spermatozoa.Scientific reports · 2025Article
- Unique Physiological Mechanisms of Sperm Storage and Prolonged Sperm Survival in Hen Oviducts: A Review.The journal of poultry science · 2025Review
- Identification of genes associated with sperm storage capacity in hens at different times after insemination by RNA-seq and Ribo-seq.BMC genomics · 2024Article
- Article
- The construction of a testis transcriptional cell atlas from embryo to adult reveals various somatic cells and their molecular roles.Journal of translational medicine · 2023Article
- Uterine microbial communities and their potential role in the regulation of epithelium cell cycle and apoptosis in aged hens.Microbiome · 2023Article
- Transcriptome identification of genes associated with uterus-vagina junction epithelial folds formation in chicken hens.Poultry science · 2023Article
- Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens.Frontiers in physiology · 2023Article
- Turkey hen sperm storage tubule transcriptome response to artificial insemination and the presence of semen.Frontiers in physiology · 2023Article
- Transcriptomic and Metabolomic Profile Analysis of Muscles Reveals Pathways and Biomarkers Involved in Flavor Differences between Caged and Cage-Free Chickens.Foods (Basel, Switzerland) · 2022Article
- Characteristics of the Mating Behavior of Domesticated Geese fromAnimals : an open access journal from MDPI · 2022Article
- Comparative transcriptome analysis of Indian domestic duck reveals candidate genes associated with egg production.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Avian sperm storage tubules (SSTs), which are located in the uterovaginal junction (UVJ) of the oviduct, are primary sperm storage sites after mating or artificial insemination. The mechanism underlying reduced sperm storage efficiency of SSTs which is highly correlated with decreased fertility rates in aged laying breeders remains largely unclear. Here, comparative transcriptomic analysis between the aged and young White Leghorn hens (120 vs. 30 wk) was applied to identify gene expression changes of UVJs containing SSTs. Bioinformatics analysis revealed 567 upregulated and 1998 downregulated differentially expressed genes. Gene ontology analysis was highly enriched in terms of immune system, cell adhesion, and cytoskeleton proteins. Kyoto Encyclopedia of Genes and Genomes analysis revealed 5 significant (P < 0.05) pathways including inositol phosphate and glycerophospholipid metabolism. β-Galactosidase staining of chicken UVJ sections suggested increased cell senescence via aging. Oil Red O staining and immunohistochemistry detection of ADFP both confirmed distribution of lipid droplets in SST cells with increased intensity in aged breeders. The lipid synthesis and metabolism-related genes represented by TFAP2 and PLD1 were differentially expressed in aged laying breeders. The upregulation of IL15 and downregulation of a large number of immune-related genes in aged breeders indicate altered immune homeostasis in UVJs and SSTs. The increased accumulation of lipids, and altered immunity homeostasis, combined with other factors (TJP1, MYL9, AFDN, and RPL13, etc.) are potentially dominant effectors to decrease the sperm storage efficiency and egg fertility in aged laying breeders.
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