ArticleMicrobiome2021
Accurate identification and quantification of commensal microbiota bound by host immunoglobulins.
Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Altered SIgA-targeting of gut microbiota is associated with long-term dysbiosis in pediatric solid organ transplant recipients.Gut microbes · 2026Article
- Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease.Cell reports. Medicine · 2026Article
- Ig-microbiota binding patterns in mothers and infants: a scoping review.Gut microbiome (Cambridge, England) · 2026Review
- Article
- Gut microbial IgA coating in infants with traditional farming lifestyle and urban infants with allergic outcomes.Frontiers in immunology · 2026Article
- Industrialization drives convergent microbial and physiological shifts in the human metaorganism.bioRxiv : the preprint server for biology · 2025Article
- Secretory IgA amplification during immune checkpoint blockade enhances the control of tumor growth by enterotropic T cells.Science advances · 2025Article
- Commensal Bacteria Drive B-cell Lymphomagenesis in the Setting of Innate Immunodeficiency.Blood cancer discovery · 2025Article
- Mucosal immune responses and intestinal microbiome associations in wild spotted hyenas (Crocuta crocuta).Communications biology · 2025Article
- Alterations in Gut Microbiome-Host Relationships After Immune Perturbation in Patients With Multiple Sclerosis.Neurology(R) neuroimmunology & neuroinflammation · 2025Article
- Myeloperoxidase gene knockout causes local inflammation and dysbiosis in the murine gut.Gut microbes reports · 2025Article
- Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metagenomic immunoglobulin sequencing reveals IgA coating of microbial strains in the healthy human gut.Nature microbiology · 2025Article
- Immunomolecular and reactivity landscapes of gut IgA subclasses in homeostasis and inflammatory bowel disease.The Journal of experimental medicine · 2024Article
- Next-generation IgA-SEQ allows for high-throughput, anaerobic, and metagenomic assessment of IgA-coated bacteria.Microbiome · 2024Article
- The interrelation between microbial immunoglobulin coating, vaginal microbiota, ethnicity, and preterm birth.Microbiome · 2024Article
- Metagenomic Immunoglobulin Sequencing (MIG-Seq) Exposes Patterns of IgA Antibody Binding in the Healthy Human Gut Microbiome.bioRxiv : the preprint server for biology · 2023Article
- Secretory IgA impacts the microbiota density in the human nose.Microbiome · 2023Article
- Investigating Antibody Reactivity to the Intestinal Microbiome in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Feasibility Study.International journal of molecular sciences · 2023Article
- Exploring Immunome and Microbiome Interplay in Reproductive Health: Current Knowledge, Challenges, and Novel Diagnostic Tools.Seminars in reproductive medicine · 2023Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundIdentifying which taxa are targeted by immunoglobulins can uncover important host-microbe interactions. Immunoglobulin binding of commensal taxa can be assayed by sorting bound bacteria from samples and using amplicon sequencing to determine their taxonomy, a technique most widely applied to study Immunoglobulin A (IgA-Seq). Previous experiments have scored taxon binding in IgA-Seq datasets by comparing abundances in the IgA bound and unbound sorted fractions. However, as these are relative abundances, such scores are influenced by the levels of the other taxa present and represent an abstract combination of these effects. Diversity in the practical approaches of prior studies also warrants benchmarking of the individual stages involved. Here, we provide a detailed description of the design strategy for an optimised IgA-Seq protocol. Combined with a novel scoring method for IgA-Seq datasets that accounts for the aforementioned effects, this platform enables accurate identification and quantification of commensal gut microbiota targeted by host immunoglobulins.
resultsUsing germ-free and Rag1
conclusionsWe provide a detailed IgA-Seq protocol to accurately isolate IgA-bound taxa from intestinal samples. Using simulated and experimental data, we demonstrate novel probability-based scores that adjust for the compositional nature of relative abundance data to accurately quantify taxon-level IgA binding. All scoring approaches are made available in the IgAScores R package. These methods should improve the generation and interpretation of IgA-Seq datasets and could be applied to study other immunoglobulins and sample types. Video abstract.
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