Evidence map›Paper›PMID 33516128›Full record

ArticleJournal of the mechanical behavior of biomedical materials2021

Biomechanical properties of acellular scar ECM during the acute to chronic stages of myocardial infarction.

Bryn L Brazile, J Ryan Butler, Sourav S Patnaik, Andrew Claude, Raj Prabhu, Lakiesha N Williams, Karla L Perez, Kytai T Nguyen, Ge Zhang, Pietro Bajona and 4 more

Abstract read
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In one paragraph

Article in Journal of the mechanical behavior of biomedical materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Bryn L BrazileDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA.
J Ryan ButlerDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA.
Sourav S PatnaikDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA.
Andrew ClaudeDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA.
Raj PrabhuDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA.
Lakiesha N WilliamsDepartment of Biological Engineering and College of Veterinary Medicine, Mississippi State University, MS, 39762, USA; Department of Biomedical Engineering, University of Florida, Gainesville, FL, 32611, USA.
Karla L PerezDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
Kytai T NguyenDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
Ge ZhangDepartment of Biomedical Engineering, University of Akron, Akron, OH, 44325, USA.
Pietro BajonaDepartment of Cardiovascular and Thoracic Surgery, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Matthias PeltzDepartment of Cardiovascular and Thoracic Surgery, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Yong YangDepartment of Biomedical Engineering, University of Northern Texas, Denton, TX, 76203, USA.
Yi HongDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
Jun LiaoDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA. Electronic address: jun.liao@uta.edu.

Funding

Interdisciplinary Training in Nanotechnology for Cardiovascular and Lung Diseases in North TexasT32HL134613 · NHLBI · UNIVERSITY OF TEXAS ARLINGTON · PI NGUYEN, KYTAI TRUONG · 2017 to 2021
$1.1M
CONTROL OF CARDIAC FIBROSIS TO PREVENT CARDIAC FUNCTION DETERIORATIONR01EB022018 · NIBIB · WASHINGTON UNIVERSITY · PI GUAN, JIANJUN · 2016 to 2018
$1.1M
A Controlled Septal Ablation for Inoperable Hypertrophic CardiomyopathyR15HL159599 · NHLBI · UNIVERSITY OF TEXAS ARLINGTON · PI LIAO, JUN · 2022 to 2022
$433k
NHLBI NIH HHS R15 HL159599NHLBI NIH HHS T32 HL134613NIBIB NIH HHS R01 EB022018
6 · The paper itself

Abstract

After myocardial infarction (MI), the infarcted tissue undergoes dynamic and time-dependent changes. Previous knowledge on MI biomechanical alterations has been obtained by studying the explanted scar tissues. In this study, we decellularized MI scar tissue and characterized the biomechanics of the obtained pure scar ECM. By thoroughly removing the cellular content in the MI scar tissue, we were able to avoid its confounding effects. Rat MI hearts were obtained from a reliable and reproducible model based on permanent left coronary artery ligation (PLCAL). MI heart explants at various time points (15 min, 1 week, 2 weeks, 4 weeks, and 12 weeks) were subjected to decellularization with 0.1% sodium dodecyl sulfate solution for ~1-2 weeks to obtain acellular scar ECM. A biaxial mechanical testing system was used to characterize the acellular scar ECM under physiologically relevant loading conditions. After decellularization, large decrease in wall thickness was observed in the native heart ECM and 15 min scar ECM, implying the collapse of cardiomyocyte lacunae after removal of heart muscle fibers. For scar ECM 1 week, 2 weeks, and 4 weeks post infarction, the decrease in wall thickness after decellularization was small. For scar ECM 12 weeks post infarction, the reduction amount of wall thickness due to decellularization was minimal. We found that the scar ECM preserved the overall mechanical anisotropy of the native ventricle wall and MI scar tissue, in which the longitudinal direction is more extensible. Acellular scar ECM from 15 min to 12 weeks post infarction showed an overall stiffening trend in biaxial behavior, in which longitudinal direction was mostly affected and manifested with a decreased extensibility and increased modulus. This reduction trend of longitudinal extensibility also led to a decreased anisotropy index in the scar ECM from the acute to chronic stages of MI. The post-MI change in biomechanical properties of the scar ECM reflected the alterations of collagen fiber network, confirmed by the histology of scar ECM. In short, the reported structure-property relationship reveals how scar ECM biophysical properties evolve from the acute to chronic stages of MI. The obtained information will help establish a knowledge basis about the dynamics of scar ECM to better understand post-MI cardiac remodeling.

Indexed as

CicatrixMyocardial InfarctionAnimalsExtracellular MatrixHeart VentriclesMyocardiumMyocytes, CardiacRatsVentricular RemodelingBiaxial mechanical testingCollagen fibersExtracellular matrixMyocardial infarctionRat heartsScar tissue

Identifiers

PMID33516128
PMCPMC8245054

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.