ArticleLab on a chip2021
Controllable fusion of human brain organoids using acoustofluidics.
Article in Lab on a chip, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- Mapping the Cerebral Organoid Landscape: A Systematic Review of Preclinical 3D Models in Neuroscience.Advanced healthcare materials · 2026Pooled it
- A wearable acoustofluidic patch for rapid reversal of opioid overdose.Nature communications · 2026Article
- Spatially defined axonal guidance in neural organoids with micropatterned microfluidic channels.bioRxiv : the preprint server for biology · 2026Article
- Organoid Brain-Machine-Interface Devices for Central Nervous System Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Engineering Human Retinal Organoids and Eye-on-a-Chip Models for Degenerative Eye Disease.ACS biomaterials science & engineering · 2026Review
- Article
- Engineering human neuronal diversity: Morphogens and stem cell technologies for neurodevelopmental biology.Stem cell reports · 2025Review
- Bioengineering gradients for controlled embryo and organ modeling.Current opinion in biomedical engineering · 2025Article
- Understanding monocyte-driven neuroinflammation in Alzheimer's disease using human cortical organoid microphysiological systems.Science advances · 2025Article
- Brain organoids: building higher-order complexity and neural circuitry models.Trends in biotechnology · 2025Review
- An acoustic levitation platform for high-content histological analysis of 3D tissue culture.Lab on a chip · 2025Article
- Human brain organoids for understanding substance use disorders.Drug metabolism and pharmacokinetics · 2025Review
- Acoustic virtual 3D scaffold for direct-interacting tumor organoid-immune cell coculture systems.Science advances · 2024Article
- Engineering human midbrain organoid microphysiological systems to model prenatal PFOS exposure.The Science of the total environment · 2024Article
- Multiscale engineering of brain organoids for disease modeling.Advanced drug delivery reviews · 2024Review
- The Role of Biophysical Factors in Organ Development: Insights from Current Organoid Models.Bioengineering (Basel, Switzerland) · 2024Review
- Design of neural organoids engineered by mechanical forces.IBRO neuroscience reports · 2024Review
- Ultrasound-induced reorientation for multi-angle optical coherence tomography.Nature communications · 2024Article
- Exploiting Sound for Emerging Applications of Extracellular Vesicles.Nano research · 2024Article
- Brain organoid methodologies to explore mechanisms of disease in progressive multiple sclerosis.Frontiers in cellular neuroscience · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The fusion of human organoids holds promising potential in modeling physiological and pathological processes of tissue genesis and organogenesis. However, current fused organoid models face challenges of high heterogeneity and variable reproducibility, which may stem from the random fusion of heterogeneous organoids. Thus, we developed a simple and versatile acoustofluidic method to improve the standardization of fused organoid models via a controllable spatial arrangement of organoids. By regulating dynamic acoustic fields within a hexagonal acoustofluidic device, we can rotate, transport, and fuse one organoid with another in a contact-free, label-free, and minimal-impact manner. As a proof-of-concept to model the development of the human midbrain-to-forebrain mesocortical pathway, we acoustically fused human forebrain organoids (hFOs) and human midbrain organoids (hMOs) with the controllable alignment of neuroepithelial buds. We found that post-assembly, hMO can successfully project tyrosine hydroxylase neurons towards hFO, accompanied by an increase of firing rates and synchrony of excitatory neurons. Moreover, we found that our controllable fusion method can regulate neuron projection (e.g., range, length, and density), projection maturation (e.g., higher firing rate and synchrony), and neural progenitor cell (NPC) division in the assembloids via the initial spatial control. Thus, our acoustofluidic method may serve as a label-free, contact-free, and highly biocompatible tool to effectively assemble organoids and facilitate the standardization and robustness of organoid-based disease models and tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.