ArticleCommunications biology2021
Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Venous Nanoflap Oscillations: Biomechanical Determinants and Hydrodynamic Consequences in the Deep Cerebral Venous System.International journal of molecular sciences · 2026Review
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.bioRxiv : the preprint server for biology · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Lipid droplet-mitochondria contact sites as druggable spatial-pharmacology targets in respiratory disease: cross-cell-type mechanisms and translational strategies.Frontiers in cell and developmental biology · 2026Review
- Structural Characterization of Protein-Nucleic Acid Complexes: An Overview of the Recent Innovation in the Analytic Methods.International journal of molecular sciences · 2025Review
- Visualization of lysosomal membrane proteins by cryo electron tomography.Nature communications · 2025Article
- Review
- Alveolar epithelial paxillin in postnatal lung alveolar development.Biology open · 2025Article
- Flotillins in membrane trafficking and physiopathology.Biology of the cell · 2025Review
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Exploring Transient States of PAmKate to Enable Improved Cryogenic Single-Molecule Imaging.Journal of the American Chemical Society · 2024Article
- Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging.bioRxiv : the preprint server for biology · 2024Article
- Structure of the flotillin complex in a native membrane environment.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Correlative Light and Electron Cryo-Microscopy Workflow Combining Micropatterning, Ice Shield, and an In-Chamber Fluorescence Light Microscope.Bio-protocol · 2023Article
- Correlative montage parallel array cryo-tomography for in situ structural cell biology.Nature methods · 2023Article
- Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET.bioRxiv : the preprint server for biology · 2023Article
- The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion.The EMBO journal · 2023Article
- Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology.Nature methods · 2023Review
- ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study.Nature methods · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lamellar bodies (LBs) are surfactant-rich organelles in alveolar cells. LBs disassemble into a lipid-protein network that reduces surface tension and facilitates gas exchange in the alveolar cavity. Current knowledge of LB architecture is predominantly based on electron microscopy studies using disruptive sample preparation methods. We established and validated a post-correlation on-lamella cryo-correlative light and electron microscopy approach for cryo-FIB milled cells to structurally characterize and validate the identity of LBs in their unperturbed state. Using deconvolution and 3D image registration, we were able to identify fluorescently labeled membrane structures analyzed by cryo-electron tomography. In situ cryo-electron tomography of A549 cells as well as primary Human Small Airway Epithelial Cells revealed that LBs are composed of membrane sheets frequently attached to the limiting membrane through "T"-junctions. We report a so far undescribed outer membrane dome protein complex (OMDP) on the limiting membrane of LBs. Our data suggest that LB biogenesis is driven by parallel membrane sheet import and by the curvature of the limiting membrane to maximize lipid storage capacity.
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