ArticleScientific reports2021
Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Rare Case of Cutaneous Infection by Corynascus verrucosus in a Shih Tzu Dog: Molecular Identification and Phylogenetic Analysis.Veterinary medicine and science · 2026Article
- Clinical Significance of Intestinal Fungal Overgrowth: Integrating the Gut Mycobiome into Modern Gastroenterology.Microorganisms · 2026Review
- Fungal dynamics and lignocellulose depolymerization in Pinus koraiensis: impact of longhorn beetle infestation.BMC microbiology · 2025Article
- FungID: Innovative Fungi Identification Method with Chromogenic Profiling of Colony Color Patterns.Pathogens (Basel, Switzerland) · 2025Article
- Root Microbiome and Metabolome Traits Associated with Improved Post-Harvest Root Storage for Sugar Beet Breeding Lines Under Southern Idaho Conditions.International journal of molecular sciences · 2024Article
- Exploring fungal diversity in Vietnam: A citizen science initiative.One health (Amsterdam, Netherlands) · 2024Article
- Fungal Spore Richness and Abundance of Allergenic Taxa: Comparing a Portable Impactor and Passive Trap Indoors and Outdoors in an Urban Setting.Microbial ecology · 2024Article
- Ridge planting increases the rhizosphere microbiome diversity and improves the yield of Pinellia ternata (Thunb.) Breit in North China.PloS one · 2024Article
- Management of Fusarium Wilt Disease of Maize (Zea mays L.) with Selected Antimycotic Plant Extracts and Inhibitory Bacillus Strains.Current microbiology · 2023Article
- Mind the gap between non-activated (non-aggressive) and activated (aggressive) indoor fungal testing: impact of pre-sampling environmental settings on indoor air readings.UCL open. Environment · 2023Article
- Microbial Contamination in the Coffee Industry: An Occupational Menace besides a Food Safety Concern?International journal of environmental research and public health · 2022Article
- Current Insight into Traditional and Modern Methods in Fungal Diversity Estimates.Journal of fungi (Basel, Switzerland) · 2022Review
- Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities.Journal of fungi (Basel, Switzerland) · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture-independent DNA sequencing of fungal internal transcribed spacer 2 (ITS2) region was compared to a culture-dependent morphological identification technique to characterize house dust-borne fungal communities. The abundant genera were Aspergillus, Wallemia, Cladosporium, and Penicillium. Statistically significant between-method correlations were observed for Wallemia and Cladosporium (Spearman's ρ = 0.75 and 0.72, respectively; p < 0.001). Penicillium tended to be detected with much higher (averaged 26-times) relative abundances by the culture-based method than by the DNA-based method, although statistically significant inter-method correlation was observed with Spearman's ρ = 0.61 (p = 0.002). Large DNA sequencing-based relative abundances observed for Alternaria and Aureobasidium were likely due to multicellularity of their spores with large number of per-spore ITS2 copies. The failure of the culture-based method in detectiing Toxicocladosporium, Verrucocladosporium, and Sterigmatomyces was likely due to their fastidiousness growth on our nutrient medium. Comparing between the two different techniques clarified the causes of biases in identifying environmental fungal communities, which should be amended and/or taken into consideration when the methods are used for future fungal ecological studies.
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