ArticleScientific reports2021
Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Multifunctional Agarose-Based Biomaterials: From Tissue Engineering and Immunomodulation to Advanced Diagnostics and Translational Applications.Gels (Basel, Switzerland) · 2026Review
- A Tissue Engineering's Guide to Biomimicry.Macromolecular bioscience · 2025Review
- A Comprehensive Guide to Enzyme Immobilization: All You Need to Know.Molecules (Basel, Switzerland) · 2025Review
- Unlocking the Potential of Stem Cell Microenvironments In Vitro.Bioengineering (Basel, Switzerland) · 2024Review
- Platelet-Rich Plasma for Degenerative Spine Disease: A Brief Overview.Spine surgery and related research · 2024Review
- Self-Assembling Peptides with Insulin-Like Growth Factor Mimicry.ACS applied materials & interfaces · 2024Article
- Polysaccharide-based hydrogel enriched by epidermal growth factor peptide fragment for improving the wound healing process.Heliyon · 2023Article
- Growth Factor Immobilization to Synthetic Hydrogels: Bioactive bFGF-Functionalized Polyisocyanide Hydrogels.Advanced healthcare materials · 2023Article
- In Vitro and In Vivo Evaluation of Epidermal Growth Factor (EGF) Loaded Alginate-Hyaluronic Acid (AlgHA) Microbeads System for Wound Healing.Journal of functional biomaterials · 2023Article
- Article
- Changes in the Transcriptome Profiles of Human Amnion-Derived Mesenchymal Stromal/Stem Cells Induced by Three-Dimensional Culture: A Potential Priming Strategy to Improve Their Properties.International journal of molecular sciences · 2022Article
- Polymeric Biomaterials for the Treatment of Cardiac Post-Infarction Injuries.Pharmaceutics · 2021Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC-MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.
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