ArticleInsects2021
Five Silkworm 30K Proteins Are Involved in the Cellular Immunity against Fungi.
Article in Insects, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- TheGenes · 2024Article
- LP30K protein manifested in hemocytes of3 Biotech · 2023Article
- Article
- Vitellogenin receptor transports the 30K protein LP1 without cell-penetrating peptide, into the oocytes of the silkworm,Frontiers in physiology · 2023Article
- Hemocyte Clusters Defined by scRNA-Seq inFrontiers in immunology · 2022Article
- Effects of Cordycepin inMicroorganisms · 2021Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
background30K proteins are a major group of nutrient storage proteins in the silkworm hemolymph. Previous studies have shown that 30K proteins are involved in the anti-fungal immunity; however, the molecular mechanism involved in this immunity remains unclear.
methodsWe investigated the transcriptional expression of five 30K proteins, including BmLP1, BmLP2, BmLP3, BmLP4, and BmLP7. The five recombinant 30K proteins were expressed in an
resultsThe transcriptional expression showed that the five 30K proteins were significantly upregulated after injection of pathogen-associated molecular patterns to the fifth instar larvae, indicating the possibility of their involvement in immune response. The binding assay showed that only BmLP1 and BmLP4 can bind to both fungal cells and silkworm hemocytes. Furthermore, we found that BmLP1-coated and BmLP4-coated agarose beads promote encapsulation of hemocytes in vitro. The hemocyte encapsulation was blocked when the BmLP1-coated beads were preincubated with BmLP1 specific polyclonal antibodies.
conclusionsThese results demonstrate that 30K proteins are involved in the cellular immunity of silkworms by acting as pattern recognition molecules to directly recruit hemocytes to the fungal surface.
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Registered trials
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