ArticleThe Science of the total environment2021
The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers, 2 of them syntheses that pooled it.
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Who cites it
80 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Wastewater surveillance to infer COVID-19 transmission: A systematic review.The Science of the total environment · 2022Pooled it
- Accounting for methodological variabilities in disease outbreak surveillance using wastewater-based epidemiology: a case study with SARS-CoV-2.Analytical and bioanalytical chemistry · 2026Article
- Remotely Controlled Continuous Surveillance of Viral RNA in Wastewater Using a LoRa Network.IEEE internet of things journal · 2026Article
- Implementation of SARS-CoV-2 Wastewater Surveillance Systems in Germany-Pilot Study in the Federal State of Thuringia.Microorganisms · 2026Article
- Wastewater-Based Surveillance of SARS-CoV-2 and Modeling of COVID-19 Infection Trends.Tropical medicine and infectious disease · 2025Article
- Wastewater based genomic surveillance key to population level monitoring of AmpC/ESBL producing Escherichia coli.Scientific reports · 2025Article
- Tracking the Spread of the BA.2.86 Lineage in Italy Through Wastewater Analysis.Food and environmental virology · 2024Article
- Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater.Microorganisms · 2024Article
- Detection of human enteric viral genes in a non-native winter crane fly, Trichocera maculipennis (Diptera) in the sewage treatment facilities at Antarctic stations.Parasites & vectors · 2024Article
- The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater.Scientific reports · 2024Article
- The comparison of decay rates of infectious SARS-CoV-2 and viral RNA in environmental waters and wastewater.The Science of the total environment · 2024Article
- Comparison of Different Reverse Transcriptase-Polymerase Chain Reaction-Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study.JMIR public health and surveillance · 2024Article
- Persistence of human respiratory viral RNA in wastewater-settled solids.Applied and environmental microbiology · 2024Article
- Detection of SARS-COV-2 variants and their proportions in wastewater samples using next-generation sequencing in Finland.Scientific reports · 2024Article
- Optimal Selection of Sampling Points within Sewer Networks for Wastewater-Based Epidemiology Applications.Methods and protocols · 2024Article
- Long-term monitoring of COVID-19 prevalence in raw and treated wastewater in Salvador, the largest capital of the Brazilian Northeast.Scientific reports · 2023Article
- Multiplex Assays Enable Simultaneous Detection and Identification of SARS-CoV-2 Variants of Concern in Clinical and Wastewater Samples.ACS measurement science au · 2023Article
- SARS-CoV-2 in the environment: Contamination routes, detection methods, persistence and removal in wastewater treatment plants.The Science of the total environment · 2023Review
- Patterns of SARS-CoV-2 circulation revealed by a nationwide sewage surveillance programme, the Netherlands, August 2020 to February 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used to monitor the population-level prevalence of the COVID-19 disease. In many cases, due to lockdowns or analytical delays, the analysis of wastewater samples might only be possible after prolonged storage. In this study, the effect of storage conditions on the RNA copy numbers of the SARS-CoV-2 virus in wastewater influent was studied and compared to the persistence of norovirus over time at 4 °C, -20 °C, and -75 °C using the reverse-transcription quantitative PCR (RT-qPCR) assays E-Sarbeco, N2, and norovirus GII. For the first time in Finland, the presence of SARS-CoV-2 RNA was tested in 24 h composite influent wastewater samples collected from Viikinmäki wastewater treatment plant, Helsinki, Finland. The detected and quantified SARS-CoV-2 RNA copy numbers of the wastewater sample aliquots taken during 19-20 April 2020 and stored for 29, 64, and 84 days remained surprisingly stable. In the stored samples, the SARS betacoronavirus and SARS-CoV-2 copy numbers, but not the norovirus GII copy numbers, seemed slightly higher when analyzed from the pre-centrifuged pellet-that is, the particulate matter of the influent-as compared with the supernatant (i.e., water fraction) used for ultrafiltration, although the difference was not statistically significant. Furthermore, when wastewater was spiked with SARS-CoV-2, linear decay at 4 °C was observed on the first 28 days, while no decay was visible within 58 days at -20 °C or -75 °C. In conclusion, freezing temperatures should be used for storage when immediate SARS-CoV-2 RNA analysis from the wastewater influent is not possible. Analysis of the particulate matter of the sample, in addition to the water fraction, can improve the detection frequency.
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