ArticlePathogens and disease2021
Identifying virulence determinants of multidrug-resistant Klebsiella pneumoniae in Galleria mellonella.
Article in Pathogens and disease, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- GM000287 is a conserved determinant of serum resistance and virulence in Klebsiella pneumoniae.PLoS pathogens · 2026Article
- Genomics of Hospital-Associated Brazilian Multidrug-Resistant Klebsiella pneumoniae: Abundance of Resistance and Virulence Genes and Mosaicism of the blaCurrent microbiology · 2026Article
- Rapid evolution of Klebsiella pneumoniae biofilms in vitro delineates adaptive changes selected during infection.Nature communications · 2026Article
- Current Overview and Main Challenges of the Use of Galleria mellonella in Biomedical Research.Current protocols · 2026Review
- A combined genetic and phenotypic marker approach enables precise detection of hypervirulentMicrobiology spectrum · 2026Article
- Strain-dependent contribution of the AcrAB-TolC efflux pump toMicrobiology (Reading, England) · 2025Article
- A critical role of copper homeostasis in the virulence of Klebsiella pneumoniae.Communications biology · 2025Article
- Gene dependence during mammalianMicrobial genomics · 2025Article
- Parallel within-host evolution alters virulence factors in an opportunistic Klebsiella pneumoniae during a hospital outbreak.Nature communications · 2025Article
- UnravelingmSystems · 2025Article
- Virulence profile of carbapenem-resistantMicrobiology spectrum · 2025Article
- Revealing fitness and virulence determinants of hypervirulentFrontiers in cellular and infection microbiology · 2025Article
- Staphylococcus aureus adapts to exploit collagen-derived proline during chronic infection.Nature microbiology · 2024Article
- Deciphering the gastrointestinal carriage ofInfection and immunity · 2024Review
- Transposon mutagenesis screen ineLife · 2024Article
- Review
- Transferable AmpCs inAntimicrobial agents and chemotherapy · 2024Article
- Development of a quadruple qRT-PCR assay for simultaneous identification of hypervirulent and carbapenem-resistantMicrobiology spectrum · 2024Article
- Essential gene complement of Planctopirus limnophila from the bacterial phylum Planctomycetes.Nature communications · 2023Article
- Klebsiella pneumoniae causes bacteremia using factors that mediate tissue-specific fitness and resistance to oxidative stress.PLoS pathogens · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Infections caused by Klebsiella pneumoniae are a major public health threat. Extensively drug-resistant and even pan-resistant strains have been reported. Understanding K. pneumoniae pathogenesis is hampered by the fact that murine models of infection offer limited resolution for non-hypervirulent strains which cause the majority of infections. The insect Galleria mellonella larva is a widely used alternative model organism for bacterial pathogens. We have performed genome-scale fitness profiling of a multidrug-resistant K. pneumoniae ST258 strain during infection of G. mellonella, to determine if this model is suitable for large-scale virulence factor discovery in this pathogen. Our results demonstrated a dominant role for surface polysaccharides in infection, with contributions from siderophores, cell envelope proteins, purine biosynthesis genes and additional genes of unknown function. Comparison with a hypervirulent strain, ATCC 43816, revealed substantial overlap in important infection-related genes, as well as additional putative virulence factors specific to ST258, reflecting strain-dependent fitness effects. Our analysis also identified a role for the metalloregulatory protein NfeR (YqjI) in virulence. Overall, this study offers new insight into the infection fitness landscape of K. pneumoniae, and provides a framework for using the highly flexible and easily scalable G. mellonella infection model to dissect molecular virulence mechanisms of bacterial pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.