Evidence map›Paper›PMID 33512337›Full record

ArticleJournal of the International Academy of Periodontology2021

TLR2 and TLR4 Differentially Regulate the Osteogenic Capacity of Human Periodontal Ligament Fibroblasts.

Sujiwan Seubbuk, Rudee Surarit, Danielle Stephens, Hatice Hasturk, Thomas E Van Dyke, Alpdogan Kantarci

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of the International Academy of Periodontology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Sujiwan SeubbukMolecular Medicine Program, Faculty of Science, Mahidol University, Ratchthewi, Bangkok Thailand; Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA; and Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Ratchthewi, Bangkok Thailand.
Rudee SuraritDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Ratchthewi, Bangkok Thailand.
Danielle StephensDepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
Hatice HasturkDepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
Thomas E Van DykeDepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
Alpdogan KantarciDepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA. E-mail address: akantarci@forsyth.org.
Mahidol University · TH

Funding

Mechanisms of Resolvin E1 in Periodontal RegenerationR01DE025020 · NIDCR · ADA FORSYTH INSTITUTE, INC. · PI VAN DYKE, THOMAS ELLIOTT · 2015 to 2023
$4.9M
Neutrophils and Periodontitis in DiabetesR01DE015566 · NIDCR · BOSTON UNIVERSITY MEDICAL CAMPUS · PI VAN DYKE, THOMAS ELLIOTT · 2005 to 2014
$4.2M
Hypoxia and PMN Function in PeriodontitisR21DE020906 · NIDCR · ADA FORSYTH INSTITUTE, INC. · PI KANTARCI, IBRAHIM ALPDOGAN · 2011 to 2012
$478k
NIDCR NIH HHS R01 DE015566NIDCR NIH HHS R01 DE025020NIDCR NIH HHS R21 DE020906
6 · The paper itself

Abstract

aimsTo test that the osteogenic capacity of periodontal ligament (PDL) fibroblasts can be mediated by TLR2 and TLR4 activation. MATERIALS AND

methodsHuman PDL fibroblasts were cultured in osteogenic medium and treated with TLR2 and TLR4 agonists (Pam3CSK4 and monophosphoryl Lipid A (MPLA), respectively). Cell proliferation was measured by MTT and BrdU incorporation. Osteogenic differentiation was measured by alkaline phosphatase (ALP) activity. Nodule formation was measured for osteoblast function. The expression of markers of potential signaling pathways (RUNX2, OCN, BSP and Osterix) was evaluated by quantitative PCR.

resultsPDL fibroblasts grew at the same rate during the first 5 days in response to both Pam3CSK5 and MPLA. On day 7, cells cultured in the presence of Pam3CSK4 had a significantly higher rate of DNA replication, while cells in MPLA group had a significantly lower DNA replication rate (one-third) compared to the control (p less than 0.05). Pam3CSK4 induced significantly higher ALP activity and larger calcified nodules. TLR4 activation significantly reduced the expression of RUNX2 and osterix and enhanced OCN. Neither TLR2 nor TLR4 affected BSP expression.

conclusionsThese data suggest that the activation of TLR2 and TLR4 differentially and perhaps antagonistically modulate osteogenesis by human PDL fibroblasts and have a direct role of TLR-mediated PDL function during periodontal regeneration as a potential target for therapeutics.

Indexed as

OsteogenesisPeriodontal LigamentCells, CulturedFibroblastsHumansToll-Like Receptor 2Toll-Like Receptor 4TLR2 protein, humanTLR4 protein, humanToll-Like Receptor 2Toll-Like Receptor 4osteogenesisPeriodontal fibroblaststoll-like receptor

Identifiers

PMID33512337
PMCPMC8142849
OpenAlexW3123661549

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.