ArticleMolecular biology reports2021
Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions.
Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 30 citations in OpenAlex.
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- Genome-wide characterization of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- qPCR-Based Reference Gene Validation inCurrent issues in molecular biology · 2025Article
- Screening and validation of reference genes of Gynostemma pentaphyllum with different tissues and under different treatment conditions by RT-qPCR analysis.Molecular biology reports · 2025Article
- Reference genes selection and validation in yam by real-time quantitative polymerase chain reaction.Scientific reports · 2025Article
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- Polyethylene Glycol Priming Enhances the Seed Germination and Seedling Growth ofPlants (Basel, Switzerland) · 2024Article
- Genome-wide identification, stress- and hormone-responsive expression characteristics, and regulatory pattern analysis of Scutellaria baicalensis SbSPLs.Plant molecular biology · 2024Article
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- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
- A Novel R2R3-MYB Transcription FactorInternational journal of molecular sciences · 2022Article
- Validation of Appropriate Reference Genes for qRT-PCR Normalization in Oat (International journal of molecular sciences · 2022Article
- Systematic Analysis and Functional Characterization of R2R3-MYB Genes inInternational journal of molecular sciences · 2022Article
- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Scutellaria baicalensis Georgi is a famous medicinal plant with its dried roots having been used as a traditional Chinese medicinal for more than 2000 years. Although its genome sequence has previously been published and molecular biology methods have been used to study this species, no suitable internal reference genes have been investigated for standardization of gene expression via quantitative real-time polymerase chain reaction (qRT-PCR). Here, the stabilities of 10 candidate reference genes, ACT11, ACT7, α-TUB, β-TUB, GAPDH, UBC, RPL, SAM, HSP70, and PP2A, were analyzed by four different procedures of GeNorm, NormFinder, BestKeeper, and RefFinder. Their expression stabilities were evaluated under various conditions, including different tissue types (root, stem, leaf, and flower), hormone stimuli treatments (methyl jasmonate, salicylic acid, and abscisic acid), and abiotic stresses (heavy metal, salt, drought, cold, and wounding). The results indicated that β-TUB was the most stable gene for all tested samples, while ACT11 was the most unstable. The most stable reference gene was not consistent under different conditions. β-TUB exhibited the highest stability for different tissue types and abiotic stresses, while for hormone stimuli treatments, ACT7 showed the highest stability. To confirm the applicability of suitable reference genes, we selected to SbF6H and SbF8H as target genes to analyze their expression levels in different tissues. This study helps to the accurate quantification of the relative expression levels of interest genes in S. baicalensis via qRT-PCR analysis.
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