Evidence map›Paper›PMID 33509174›Full record

ArticleBMC veterinary research2021

Metabolomic profiles of plasma and uterine luminal fluids from healthy and repeat breeder Holstein cows.

Natsumi Funeshima, Ryotaro Miura, Taiga Katoh, Hikari Yaginuma, Takeshi Kitou, Itaru Yoshimura, Kunitoshi Konda, Seizo Hamano, Koumei Shirasuna

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Current Insights in the Repeat Breeder Cow Syndrome.Animals : an open access journal from MDPI · 2023
    Review
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Natsumi Funeshima *Department of Animal Science, Tokyo University of Agriculture, Atsugi, Kanagawa, 243-0034, Japan.
Ryotaro Miura *Department of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan.
Taiga KatohDepartment of Animal Science, Tokyo University of Agriculture, Atsugi, Kanagawa, 243-0034, Japan.
Hikari YaginumaAnimal Bio-Technology Center, Livestock Improvement Association of Japan Inc., Shinagawa, Tokyo, 135-0041, Japan.
Takeshi KitouAnimal Bio-Technology Center, Livestock Improvement Association of Japan Inc., Shinagawa, Tokyo, 135-0041, Japan.
Itaru YoshimuraFuji Animal Research Farm, Nippon Veterinary and Life Science University, Kawaguchiko, Yamanashi, 401-3338, Japan.
Kunitoshi KondaKanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa, 243-0417, Japan.
Seizo HamanoMaebashi Institute of Animal Science, Livestock Improvement Association of Japan Inc., Maebashi, Gunma, 371-0121, Japan.
Koumei ShirasunaDepartment of Animal Science, Tokyo University of Agriculture, Atsugi, Kanagawa, 243-0034, Japan. ks205312@nodai.ac.jp.ORCID http://orcid.org/0000-0001-6759-6134
Tokyo University of Agriculture · JPNippon Veterinary and Life Science University · JPInstitute of Livestock and Grassland Science · JPKanagawa Industrial Technology Center · JP

Funding

King's Together N/A
6 · The paper itself

Abstract

backgroundRepeat breeding is a critical reproductive disorder in cattle. The problem of repeat breeder cattle remains largely unmanageable due to a lack of informative biomarkers. Here, we utilized metabolomic profiling in an attempt to identify metabolites in the blood plasma and uterine luminal fluids. We collected blood and uterine fluid from repeat breeder and healthy cows on day 7 of the estrous cycle.

resultsMetabolomic analysis identified 17 plasma metabolites detected at concentrations that distinguished between the two groups, including decreased various bile acids among the repeat breeders. However, no metabolites that varied significantly were detected in the uterine luminal fluids between two groups. Among the plasma samples, kynurenine was identified as undergoing the most significant variation. Kynurenine is a metabolite produced from tryptophan via the actions of indoleamine 2,3-dioxygenase (IDO). As IDO is key for maternal immune tolerance and induced in response to interferon tau (IFNT, ruminant maternal recognition of pregnancy factor), we examined the responsiveness to IFNT on peripheral blood mononuclear cells (PBMC) isolated from healthy and repeat breeder cows. The mRNA expression of IFNT-response makers (ISG15 and MX2) were significantly increased by IFNT treatment in a dose-dependent manner in both groups. Although treatment with IFNT promoted the expression of IDO in PBMCs from both groups, it did so at a substantially reduced rate among the repeat breeder cows, suggesting that decreased levels of kynurenine may relate to the reduced IDO expression in repeat breeder cows.

conclusionsThese findings provide valuable information towards the identification of critical biomarkers for repeat breeding syndrome in cattle.

Indexed as

AnimalsBiomarkersBody FluidsCattleFemaleMetabolomicsParityReal-Time Polymerase Chain ReactionRNAUterusBiomarkersRNABile acidsIndoleamine 2,3-dioxygenaseInterferon tauRepeat breeding

Identifiers

PMID33509174
PMCPMC7842029
OpenAlexW3124258948

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.