ArticleBMC genomics2021
Telomere length de novo assembly of all 7 chromosomes and mitogenome sequencing of the model entomopathogenic fungus, Metarhizium brunneum, by means of a novel assembly pipeline.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Fungal Hydrophobins: Taxonomic Distribution, Functional Roles, Physicochemical Properties, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2026Review
- Telomere-to-telomere genome assembly of Metarhizium acridum provides insights into Metarhizium genome evolution.BMC genomics · 2026Article
- A trio-binning approach for genome assembly reveals extensive structural variation between two Cannabis cultivars: Punto Rojo and Cherry Pie.G3 (Bethesda, Md.) · 2026Article
- Discovery of a fungal Tc toxin complex and functional mycoserpin through a novel two-by-two comparative genomics approach.Frontiers in cellular and infection microbiology · 2026Article
- Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi).Journal of fungi (Basel, Switzerland) · 2025Article
- Phylogenetic Reclassification ofPathogens (Basel, Switzerland) · 2025Article
- Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling.Cell genomics · 2025Article
- Draft hybrid genome sequence of phytopathogenMicrobiology resource announcements · 2024Article
- Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity.G3 (Bethesda, Md.) · 2024Article
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- The Identification of the Mitochondrial DNA Polymerase γ (Mip1) of the Entomopathogenic FungusMicroorganisms · 2024Article
- Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species Cladobotryum mycophilum ATHUM6906.G3 (Bethesda, Md.) · 2024Article
- Frequent horizontal chromosome transfer between asexual fungal insect pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chromosome-Level Assemblies for the Pine Pitch Canker PathogenPathogens (Basel, Switzerland) · 2024Article
- Genome sequence of a EuropeanFrontiers in plant science · 2024Article
- Hybrid de novo genome assembly and comparative genomics of three different isolates of Gnomoniopsis castaneae.Scientific reports · 2023Article
- Draft Genome Sequence of Aspergillus lacticoffeatus WU-2020, a Citric Acid Producer Suitable for Solid Culture That Belongs To Aspergillus SectionMicrobiology resource announcements · 2023Article
- Mitochondrial Transcription of Entomopathogenic Fungi Reveals Evolutionary Aspects of Mitogenomes.Frontiers in microbiology · 2022Article
- Draft Genome Sequence of a NewFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMore accurate and complete reference genomes have improved understanding of gene function, biology, and evolutionary mechanisms. Hybrid genome assembly approaches leverage benefits of both long, relatively error-prone reads from third-generation sequencing technologies and short, accurate reads from second-generation sequencing technologies, to produce more accurate and contiguous de novo genome assemblies in comparison to using either technology independently. In this study, we present a novel hybrid assembly pipeline that allowed for both mitogenome de novo assembly and telomere length de novo assembly of all 7 chromosomes of the model entomopathogenic fungus, Metarhizium brunneum.
resultsThe improved assembly allowed for better ab initio gene prediction and a more BUSCO complete proteome set has been generated in comparison to the eight current NCBI reference Metarhizium spp. genomes. Remarkably, we note that including the mitogenome in ab initio gene prediction training improved overall gene prediction. The assembly was further validated by comparing contig assembly agreement across various assemblers, assessing the assembly performance of each tool. Genomic synteny and orthologous protein clusters were compared between Metarhizium brunneum and three other Hypocreales species with complete genomes, identifying core proteins, and listing orthologous protein clusters shared uniquely between the two entomopathogenic fungal species, so as to further facilitate the understanding of molecular mechanisms underpinning fungal-insect pathogenesis.
conclusionsThe novel assembly pipeline may be used for other haploid fungal species, facilitating the need to produce high-quality reference fungal genomes, leading to better understanding of fungal genomic evolution, chromosome structuring and gene regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.