Evidence map›Paper›PMID 33508174›Full record

ArticlePharmacology research & perspectives2021

β-arrestin2 recruitment at the β2 adrenergic receptor: A luciferase complementation assay adapted for undergraduate training in pharmacology.

Mattia Ferraiolo, Pauline Beckers, Nicolas Marquet, Martin Roumain, Lucie Ruiz, Nadine Dupuis, Julien Hanson, Emmanuel Hermans

Open access · goldAbstract read
In one paragraph

Article in Pharmacology research & perspectives, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Using a NanoBRET-Based Ligand-Binding Assay at the βPharmacology research & perspectives · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Mattia FerraioloNeuropharmacology Laboratory, Institute of Neuroscience, UCLouvain, Brussels, Belgium.ORCID 0000-0002-4332-9055
Pauline BeckersNeuropharmacology Laboratory, Institute of Neuroscience, UCLouvain, Brussels, Belgium.ORCID 0000-0001-9027-2525
Nicolas MarquetNeuropharmacology Laboratory, Institute of Neuroscience, UCLouvain, Brussels, Belgium.
Martin RoumainFaculty of Pharmacy and Biomedical Sciences, UCLouvain, Brussels, Belgium.ORCID 0000-0001-8915-795X
Lucie RuizFaculty of Pharmacy and Biomedical Sciences, UCLouvain, Brussels, Belgium.
Nadine DupuisLaboratory of Molecular Pharmacology, GIGA-Molecular Biology of Disease, ULiège, Liège, Belgium.
Julien HansonLaboratory of Molecular Pharmacology, GIGA-Molecular Biology of Disease, ULiège, Liège, Belgium.ORCID 0000-0001-7063-7590
Emmanuel HermansNeuropharmacology Laboratory, Institute of Neuroscience, UCLouvain, Brussels, Belgium.ORCID 0000-0002-8589-3279
Statistics Belgium · BEUniversity of Liège · BENeuroelectronics Research Flanders · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the context of pharmacology teaching, hands-on activities constitute an essential complement to theoretical lectures. Frequently, these activities consist in exposing fresh animal tissues or even living animals to selected drugs and qualitatively or quantitatively evaluating functional responses. However, technological advancements in pharmacological research and the growing concerns for animal experimentation support the need for innovative and flexible in vitro assays adapted for teaching purposes. We herein report the implementation of a luciferase complementation assay (LCA) enabling to dynamically monitor β-arrestin2 recruitment at the β

Indexed as

Biological AssayAdrenergic beta-AgonistsAdrenergic beta-Antagonistsbeta-Arrestin 2EducationHEK293 CellsHumansIsoproterenolLuciferases, FireflyPharmacologyPropranololReceptors, Adrenergic, beta-2Surveys and QuestionnairesAdrenergic beta-AgonistsAdrenergic beta-Antagonistsbeta-Arrestin 2IsoproterenolLuciferases, FireflyPropranololReceptors, Adrenergic, beta-2ArrestinG protein-coupled receptorluciferasepA2teachingundergraduateβ2 adrenergic receptor

Identifiers

PMID33508174
PMCPMC7842874
OpenAlexW3124050555

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.