ArticlePloS one2021
Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Scaffold Material-Architecture Design Rules Linking Mechanics and Early Osteogenesis in PCL/β-TCP Grid, Honeycomb, and Gyroid Lattices.ACS omega · 2026Article
- Enhanced supercritical angle localization microscopy through point spread function modeling.Biomedical optics express · 2025Article
- ADPact: A versatile sensor for ADP-F-actin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A red fluorescent lifeact marker to study actin morphology in podocytes.Scientific reports · 2025Article
- Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains.Scientific data · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- IMA fungus · 2025Review
- Evaluation of image analysis tools for the measurement of cellular morphology.Frontiers in cell and developmental biology · 2025Review
- Phalloidin-PAINT: Enhanced quantitative nanoscale imaging of F-actin.Biophysical journal · 2024Article
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Students' Experiences with the Science and Engineering Practices in a Workshop-Based Undergraduate Research Experience.Journal of chemical education · 2024Article
- Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling.Cells · 2024Article
- Quantitative Superresolution Imaging of F-Actin in the Cell Body and Cytoskeletal Protrusions Using Phalloidin-Based Single-Molecule Labeling and Localization Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Self-Assembly and Cytocompatibility of Amino Acid Conjugates Containing a Novel Water-Soluble Aromatic Protecting Group.Biomacromolecules · 2023Article
- Article
- SMITE: Single Molecule Imaging Toolbox Extraordinaire (MATLAB).Journal of open source software · 2023Article
- Eugenol Induces Apoptosis in Tongue Squamous Carcinoma Cells by Mediating the Expression of Bcl-2 Family.Life (Basel, Switzerland) · 2022Article
- A statistical mechanics model for determining the length distribution of actin filaments under cellular tensional homeostasis.Scientific reports · 2022Article
- Article
- Cytoskeletal dysregulation and neurodegenerative disease: Formation, monitoring, and inhibition of cofilin-actin rods.Frontiers in cellular neuroscience · 2022Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Visualizing actin filaments in fixed cells is of great interest for a variety of topics in cell biology such as cell division, cell movement, and cell signaling. We investigated the possibility of replacing phalloidin, the standard reagent for super-resolution imaging of F-actin in fixed cells, with the actin binding peptide 'lifeact'. We compared the labels for use in single molecule based super-resolution microscopy, where AlexaFluor 647 labeled phalloidin was used in a dSTORM modality and Atto 655 labeled lifeact was used in a single molecule imaging, reversible binding modality. We found that imaging with lifeact had a comparable resolution in reconstructed images and provided several advantages over phalloidin including lower costs, the ability to image multiple regions of interest on a coverslip without degradation, simplified sequential super-resolution imaging, and more continuous labeling of thin filaments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.