ArticlePloS one2021
Evaluation of a multiplex PCR method for the detection of porcine parvovirus types 1 through 7 using various field samples.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- First molecular detection of porcine parvovirus 5 in slaughtered pigs from Southern Vietnam.Archives of virology · 2026Article
- Infectious Agents Associated with Abortion Outbreaks in Italian Pig Farms from 2011 to 2021.Veterinary sciences · 2024Article
- Isolation and characterization of porcine parvovirus in Vietnam.Veterinary world · 2024Article
- Article
- Identification and in vitro characterization of a novel porcine parvovirus 6 in Russia.Veterinary research communications · 2024Article
- Development and application of a quadruplex real-time PCR method for Torque teno sus virus 1, Porcine circovirus type 2, pseudorabies virus, and porcine parvovirus.Frontiers in cellular and infection microbiology · 2024Article
- Development of a RPA-CRISPR/Cas12a based rapid visual detection assay for Porcine Parvovirus 7.Frontiers in veterinary science · 2024Article
- The Novel Porcine Parvoviruses: Current State of Knowledge and Their Possible Implications in Clinical Syndromes in Pigs.Viruses · 2023Review
- Emergent Molecular Techniques Applied to the Detection of Porcine Viruses.Veterinary sciences · 2023Review
- Microfluidic-LAMP chip for the point-of-care detection of gene-deleted and wild-type African swine fever viruses and other four swine pathogens.Frontiers in veterinary science · 2023Article
- Prevalence of porcine parvovirus 1 through 7 (PPV1-PPV7) and co-factor association with PCV2 and PRRSV in Korea.BMC veterinary research · 2022Article
- A Systematic Investigation Unveils High Coinfection Status of Porcine Parvovirus Types 1 through 7 in China from 2016 to 2020.Microbiology spectrum · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine parvoviruses (PPVs) are small, nonenveloped DNA viruses that are widespread in the global pig population. PPV type 1 (PPV1) is a major causative agent of reproductive failure and has been recognized since the 1960s. In recent decades, novel PPVs have been identified and designated as PPVs 2 through 7 (PPV2~PPV7). Although the epidemiological impacts of these newly recognized parvoviruses on pigs are largely unknown, continuous surveillance of these PPVs is needed. The aim of this study was to develop an improved and efficient detection tool for these PPVs and to assess the developed method with field samples. Using 7 sets of newly designed primers, a multiplex polymerase chain reaction (mPCR) protocol was developed for the simultaneous detection of the seven genotypes of PPV (PPV1~PPV7). The sensitivity of the mPCR assay was analyzed, and the detection limit was determined to be 3×103 viral copies. The assay was highly specific in detecting one or more of the viruses in various combinations in specimens. The mPCR method was evaluated with 80 serum samples, 40 lung or lymph node samples and 40 intestine or fecal samples. When applied to these samples, the mPCR method could detect the 7 viruses simultaneously, providing rapid results regarding infection and coinfection status. In conclusion, the developed mPCR assay can be utilized as an effective and accurate diagnostic tool for rapid differential detection and epidemiological surveillance of various PPVs in numerous types of field samples.
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