ArticleMicrobial biotechnology2021
Biosynthesis of cofactor-activatable iron-only nitrogenase in Saccharomyces cerevisiae.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 22 citations in OpenAlex.
- Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria.Plant molecular biology · 2026Article
- Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria.Plant biotechnology journal · 2025Article
- Iron-Integrated Nitrogen-Rich Nanocarriers Boost Symbiotic Nitrogen Fixation and Growth in Soybean (Nanomaterials (Basel, Switzerland) · 2025Article
- Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description ofMicroorganisms · 2024Article
- Organelle-dependent polyprotein designs enable stoichiometric expression of nitrogen fixation components targeted to mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The structural components of the Azotobacter vinelandii iron-only nitrogenase, AnfDKG, form a protein complex within the plant mitochondrial matrix.Plant molecular biology · 2023Article
- Trends in Biological Ammonia Production.Biotech (Basel (Switzerland)) · 2023Review
- Ammonia Production Using Bacteria and Yeast toward a Sustainable Society.Bioengineering (Basel, Switzerland) · 2023Review
- Biological nitrogen fixation and prospects for ecological intensification in cereal-based cropping systems.Field crops research · 2022Review
- A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation.Scientific reports · 2022Article
- A conformational role for NifW in the maturation of molybdenum nitrogenase P-cluster.Chemical science · 2022Article
- The Chloroplast ofInternational journal of molecular sciences · 2021Review
- Biosynthesis of cofactor-activatable iron-only nitrogenase in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Engineering nitrogenase in eukaryotes is hampered by its genetic complexity and by the oxygen sensitivity of its protein components. Of the three types of nitrogenases, the Fe-only nitrogenase is considered the simplest one because its function depends on fewer gene products than the homologous and more complex Mo and V nitrogenases. Here, we show the expression of stable Fe-only nitrogenase component proteins in the low-oxygen mitochondria matrix of S. cerevisiae. As-isolated Fe protein (AnfH) was active in electron donation to NifDK to reduce acetylene into ethylene. Ancillary proteins NifU, NifS and NifM were not required for Fe protein function. The FeFe protein existed as apo-AnfDK complex with the AnfG subunit either loosely bound or completely unable to interact with it. Apo-AnfDK could be activated for acetylene reduction by the simple addition of FeMo-co in vitro, indicating preexistence of the P-clusters even in the absence of coexpressed NifU and NifS. This work reinforces the use of Fe-only nitrogenase as simple model to engineer nitrogen fixation in yeast and plant mitochondria.
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