ReviewFrontiers in cellular and infection microbiology2020
Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.
Review in Frontiers in cellular and infection microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Development of a NovelJournal of fungi (Basel, Switzerland) · 2026Article
- A Quantitative Real-Time PCR Assay for Detection and Quantification of the Ginseng Alternaria Leaf and Stem Blight PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- Morphological and molecular identification of Fusarium wilt resistance in tomato cultivars mediated by defense gene response.Scientific reports · 2026Article
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- A Multi-assay molecular toolkit for rapid and sensitive detection of Ustilaginoidea virens the causative agent of rice false smut using PCR, qPCR, RPA, and LAMP assays.World journal of microbiology & biotechnology · 2026Article
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- The molecular revolution in fungal diagnostics: bridging gaps across clinical, agricultural, and environmental mycology.Mycology · 2026Review
- Editorial for Special Issue "Phytopathogens: Detection and Control".Microorganisms · 2025Article
- Development and comparative evaluation of LAMP, nested PCR and Real-time PCR assays for detecting Fusarium tricinctum, a fungal pathogen of Zanthoxylum bungeanum.BMC microbiology · 2025Article
- Polyphasic characterization and mating type allele distribution of Monilinia laxa in Iranian stone fruit orchards.Scientific reports · 2025Article
- Design and Validation of Specific qPCR Primers for Soil-Borne and Apple Tree-Associated Phytopathogenic Fungi.The plant pathology journal · 2025Article
- Essential Oils as an Antifungal Alternative to Control Several Species of Fungi Isolated fromMicroorganisms · 2025Article
- Molecular virulotyping and advancing the detection of Magnaporthe oryzae using a CRISPR-Cas12a-based diagnostic tool.World journal of microbiology & biotechnology · 2025Article
- Plant Fungal Diseases and Crop Protection.Journal of fungi (Basel, Switzerland) · 2025Article
- A portable, nanopore-based genotyping platform for near real-time detection of Puccinia graminis f. sp. tritici lineages and fungicide sensitivity.BMC genomics · 2025Article
- Evolutionary genomics reveals variation in structure and genetic content implicated in virulence and lifestyle in the genus Gaeumannomyces.BMC genomics · 2025Article
- Advanced rDNA-Based Detection of Wheat Pathogens in Grain Samples Using Next-Generation Sequencing (NGS).Pathogens (Basel, Switzerland) · 2025Article
- Addition of two new genera-Frontiers in cellular and infection microbiology · 2025Article
- Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees.PloS one · 2025Article
- Pathogenic fungi (Sordariomycetes) associated with annual and perennial crops in Northern Thailand.MycoKeys · 2025Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.
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