Evidence map›Paper›PMID 33505551›Full record

ArticleWorld journal of emergency medicine2021

MicroRNA-145 plays a role in mitochondrial dysfunction in alveolar epithelial cells in lipopolysaccharide-induced acute respiratory distress syndrome.

Yi Han, Su-Cheng Mu, Jian-Li Wang, Wei Wei, Ming Zhu, Shi-Lin Du, Min Min, Yun-Jie Xu, Zhen-Ju Song, Chao-Yang Tong

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of emergency medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Yi HanEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Su-Cheng MuEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Jian-Li WangEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Wei WeiEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Ming ZhuEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Shi-Lin DuEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Min MinEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Yun-Jie XuEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhen-Ju SongEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Chao-Yang TongEmergency Department, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Fudan University · CNZhongshan Hospital · CNThe First Affiliated Hospital, Sun Yat-sen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAcute respiratory distress syndrome (ARDS) causes substantial mortalities. Alveolar epithelium is one of the main sites of cell injuries in ARDS. As an important kind of microRNAs (miRNAs), microRNA-145 (miR-145) has been studied in various diseases, while its role in ARDS has not been investigated.

methodsLipopolysaccharide (LPS) was intratracheally instilled to establish a rat ARDS model. Cytokines from bronchoalveolar lavage fluid (BALF) were measured using rat tumor necrosis factor-α and interleukin-6 enzyme-linked immunosorbent assay kits (R&D Systems), and the pathological structures were evaluated using hematoxylin and eosin (H&E) staining and transmission electron microscope; the lung miR-145 messenger RNA (mRNA) was detected using quantitative polymerase chain reaction. Bioinformatics focused on the target genes and possible pathways of gene regulation.

resultsA rat model of LPS-induced ARDS was successfully established. The miR-145 was down-regulated in the LPS-induced ARDS lung, and mitochondrial dysfunction was observed in alveolar epithelial cells, most obviously at 72 hours after LPS. TargetScan and miRDB databases were used to predict the target genes of miR-145. A total of 428 overlapping genes were identified, seven genes were associated with mitochondrial function, and

conclusionsThe miR-145 is down-regulated in LPS-induced ARDS, and affects its downstream genes targeting mitochondrial functions.

Indexed as

Acute respiratory distress syndromeLipopolysaccharideMicroRNA-145Mitochondrial functionRats

Identifiers

PMID33505551
PMCPMC7790714
OpenAlexW3113964386

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.