Evidence map›Paper›PMID 33502700›Full record

ArticleMolecular biology reports2021

Isolation and molecular characterization of MYB60 in Solanum lycopersicum.

Amparo Rodríguez-Hoces de la Guardia, María Beatriz Ugalde, Valeria Lobos-Diaz, Jesús Lucina Romero-Romero, Carlos Meyer-Regueiro, Claudio Inostroza-Blancheteau, Marjorie Reyes-Diaz, Felipe Aquea, Patricio Arce-Johnson

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Identification of the passion fruit (Frontiers in plant science · 2023
    Article
  3. Article
  4. Article
  5. Anthocyanin Biosynthesis Genes as Model Genes for Genome Editing in Plants.International journal of molecular sciences · 2021
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 2 countries.

Amparo Rodríguez-Hoces de la GuardiaFacultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.
María Beatriz UgaldeFacultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.
Valeria Lobos-DiazFacultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.
Jesús Lucina Romero-RomeroInstituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, San Joachín, Guasave, Sinaloa, Mexico.
Carlos Meyer-RegueiroFacultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.
Claudio Inostroza-BlancheteauNúcleo de Investigación en Producción Alimentaria, Departamento de Ciencias Agropecuarias y Acuícolas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile.
Marjorie Reyes-DiazDepartamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, Chile.
Felipe AqueaLaboratorio de Bioingeniería, Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Santiago, Chile.
Patricio Arce-JohnsonFacultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile. parce@bio.puc.cl.ORCID http://orcid.org/0000-0003-3854-1286
Pontificia Universidad Católica de Chile · CLAdolfo Ibáñez University · CLInstituto Politécnico Nacional · MXUniversidad Andrés Bello · CLUniversidad Católica de Temuco · CLUniversidad de La Frontera · CL

Funding

FONDECYT 1150220
6 · The paper itself

Abstract

Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.

Indexed as

DroughtsAbscisic AcidAdaptation, PhysiologicalArabidopsis ProteinsGene Expression Regulation, PlantPlants, Genetically ModifiedRegulatory Sequences, Nucleic AcidSolanum lycopersicumTranscription FactorsAbscisic AcidArabidopsis ProteinsMYB60 protein, ArabidopsisTranscription FactorsAbiotic stressDroughtMYB60Stomatal apertureTomato

Identifiers

PMID33502700
OpenAlexW3122229161

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.