ArticleBioprocess and biosystems engineering2021
Efficient brazzein production in yeast (Kluyveromyces lactis) using a chemically defined medium.
Article in Bioprocess and biosystems engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Microbial Production of Alternative Proteins for Food Applications: Advances, Industrial Challenges, and Regulatory Status.Comprehensive reviews in food science and food safety · 2026Review
- Biosynthesis and metabolic engineering of natural sweeteners.AMB Express · 2025Review
- Novel gnd_v2 Fusion Tag and Engineered TEV Protease Enable Efficient Production of Brazzein.Journal of microbiology and biotechnology · 2024Article
- A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display inACS synthetic biology · 2024Article
- Development of chemically defined media for3 Biotech · 2023Article
- Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts.Foods (Basel, Switzerland) · 2023Review
- Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature.Microbial cell factories · 2023Article
- Bioprospecting and biotechnological insights into sweet-tasting proteins by microbial hosts-a review.Bioengineered · 2022Review
- Yeast Protein as an Easily Accessible Food Source.Metabolites · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The sweet-tasting protein brazzein offers considerable potential as a functional sweetener with antioxidant, anti-inflammatory, and anti-allergic properties. Here, we optimized a chemically defined medium to produce secretory recombinant brazzein in Kluyveromyces lactis, with applications in mass production. Compositions of defined media were investigated for two phases of fermentation: the first phase for cell growth, and the second for maximum brazzein secretory production. Secretory brazzein expressed in the optimized defined medium exhibited higher purity than in the complex medium; purification was by ultrafiltration using a molecular weight cutoff, yielding approximately 107 mg L
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33502625What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.