Evidence map›Paper›PMID 33502586›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2021

VIP modulates human macrophages phenotype via FPRL1 via activation of RhoA-GTPase and PLC pathways.

Zeina Harhous, Wissam H Faour, Nabil El Zein

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In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Zeina Harhous *Gilbert and Rose-Marie Chagoury School of Medicine, Lebanese American University, P.O. Box 36, Byblos, Lebanon.
Wissam H Faour *Gilbert and Rose-Marie Chagoury School of Medicine, Lebanese American University, P.O. Box 36, Byblos, Lebanon.
Nabil El ZeinDepartment of Chemistry and Biochemistry, Faculty of Sciences, Lebanese University, Rafic Hariri University Campus Hadath, Branch 1, Beirut, Lebanon. nabilzein@ul.edu.lb.ORCID http://orcid.org/0000-0002-8596-5359
Lebanese American University · LBLebanese University · LB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

OBJECTIVE AND

designThis study is aimed at uncovering the signaling pathways activated by vasoactive intestinal peptide in human macrophages MATERIALS: Human peripheral blood mononuclear cell-derived macrophages were used for the in vitro investigation of the VIP-activated signaling pathways. METHODS AND TREATMENT: Time-course and dose-response experiments and siRNA were used in human macrophages co-challenged with various concentrations of VIP and different MAPK pharmacologic inhibitors to investigate signaling pathways activated by VIP. Flow analysis was performed to assess the levels of CD11b, CD35 and CD66. Luminescence spectrometry was used to measure the levels of the released hydrogen peroxide and the intracellular calcium levels in the media.

resultsMacrophages incubated with VIP showed increased phospho-AKT and phospho-ERK1/2 levels in a GTP-RhoA-GTPase-dependent manner. Similarly, VIP increased intracellular release of H

conclusionVIP/FPRL1/VPAC/GTP-RhoA-GTPase signaling modulated macrophages phenotype through activation of multiple signaling pathways including ERK1/2, AKT, P38, ROS, cAMP and calcium.

Indexed as

CalciumExtracellular Signal-Regulated MAP KinasesHumansHydrogen PeroxideMacrophagesMatrix Metalloproteinase 9p38 Mitogen-Activated Protein KinasesPhenotypeProto-Oncogene Proteins c-aktReceptors, Formyl PeptideReceptors, LipoxinrhoA GTP-Binding ProteinSignal TransductionType C PhospholipasesVasoactive Intestinal PeptideCalciumExtracellular Signal-Regulated MAP KinasesFPR2 protein, humanHydrogen PeroxideMatrix Metalloproteinase 9MMP9 protein, humanp38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-aktReceptors, Formyl PeptideReceptors, LipoxinrhoA GTP-Binding ProteinRHOA protein, humanType C PhospholipasesVasoactive Intestinal PeptideFPRL1MacrophageMAPKRho kinaseVIP

Identifiers

PMID33502586
OpenAlexW3125708383

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.