ArticleJournal of molecular endocrinology2021
ACOX1, regulated by C/EBPα and miR-25-3p, promotes bovine preadipocyte adipogenesis.
Article in Journal of molecular endocrinology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 26 citations in OpenAlex.
- Fructose impairs brown adipogenesis by promoting thyroid hormone resistance in differentiated brown adipocytes.Biology open · 2026Article
- Genome-Wide Association Studies of Growth and Carcass Traits in Charolais Cattle Based on High-Coverage Whole-Genome Resequencing.International journal of molecular sciences · 2025Article
- Activation of Focal Adhesion Pathway byAnimals : an open access journal from MDPI · 2025Article
- CIDEB promotes lipid deposition in goat intramuscular adipocytes.Animal bioscience · 2025Article
- Copy Number Variation and Selection Signal: Exploring the Domestication History and Phenotype Differences Between Duroc and the Chinese Native Ningxiang Pigs.International journal of molecular sciences · 2024Article
- Genome-Wide Analysis Reveals Copy Number Variant GeneAnimals : an open access journal from MDPI · 2024Article
- Identification of central regulators related to abdominal fat deposition in chickens based on weighted gene co-expression network analysis.Poultry science · 2024Article
- Integrated meta-omics reveals the regulatory landscape involved in lipid metabolism between pig breeds.Microbiome · 2024Article
- Proteomics reveals defective peroxisomal fatty acid oxidation during the progression of acute kidney injury and repair.Heliyon · 2023Article
- Effect of ACADL on the differentiation of goat subcutaneous adipocyte.Animal bioscience · 2023Article
- Article
- Identification of crucial modules and genes associated with backfat tissue development by WGCNA in Ningxiang pigs.Frontiers in genetics · 2023Article
- A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle.BMC genomics · 2022Article
- Dietary Choline Alleviates High-Fat Diet-Induced Hepatic Lipid Dysregulation via UPRmt Modulated by SIRT3-Mediated mtHSP70 Deacetylation.International journal of molecular sciences · 2022Article
- Review
- Comprehensive Analysis and Identification of Key Driver Genes for Distinguishing Between Esophageal Adenocarcinoma and Squamous Cell Carcinoma.Frontiers in cell and developmental biology · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acyl-coenzyme A oxidase 1 (ACOX1) is the first and rate-limiting enzyme in peroxisomal fatty acid β-oxidation of fatty acids. Previous studies have reported that ACOX1 was correlated with the meat quality of livestock, while the role of ACOX1 in intramuscular adipogenesis of beef cattle and its transcriptional and post-transcriptional regulatory mechanisms remain unclear. In the present study, gain-of-function and loss-of-function assays demonstrated that ACOX1 positively regulated the adipogenesis of bovine intramuscular preadipocytes. The C/EBPα-binding sites in the bovine ACOX1 promoter region at -1142 to -1129 bp, -831 to -826 bp, and -303 to -298 bp were identified by promoter deletion analysis and site-directed mutagenesis. Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) further showed that these three regions are C/EBPα-binding sites, both in vitro and in vivo, indicating that C/EBPα directly interacts with the bovine ACOX1 promoter and inhibits its transcription. Furthermore, the results from bioinformatics analysis, dual luciferase assay, site-directed mutagenesis, qRT-PCR, and Western blotting demonstrated that miR-25-3p directly targeted the ACOX1 3'UTR (3'UTR). Taken together, our findings suggest that ACOX1, regulated by transcription factor C/EBPα and miR-25-3p, promotes adipogenesis of bovine intramuscular preadipocytes via regulating peroxisomal fatty acid β-oxidation.
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