Evidence map›Paper›PMID 33501462›Full record

ArticlemedRxiv : the preprint server for health sciences2021

Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assays.

Sukalyani Banik, Kaheerman Saibire, Shraddha Suryavanshi, Glenn Johns, Soumitesh Chakravorty, Robert Kwiatkowski, David Alland, Padmapriya Banada

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Sukalyani BanikPublic Health Research Institute, 225 Warren Street, Newark, NJ 07103.
Kaheerman SaibirePublic Health Research Institute, 225 Warren Street, Newark, NJ 07103.
Shraddha SuryavanshiPublic Health Research Institute, 225 Warren Street, Newark, NJ 07103.
Glenn JohnsCepheid, Sunnyvale, CA.
Soumitesh ChakravortyCepheid, Sunnyvale, CA.
Robert KwiatkowskiCepheid, Sunnyvale, CA.
David Alland *Public Health Research Institute, 225 Warren Street, Newark, NJ 07103.
Padmapriya Banada *Public Health Research Institute, 225 Warren Street, Newark, NJ 07103.

Funding

TB detection in children using a novel stool-based GeneXpert assayR01AI131617 · NIAID · RBHS-NEW JERSEY MEDICAL SCHOOL · PI ALLAND, DAVID, NICOL, MARK PATRICK · 2018 to 2022
$2.9M
NIAID NIH HHS R01 AI131617
6 · The paper itself

Abstract

backgroundUpper respiratory samples used to test for SARS-CoV-2 virus may be infectious and present a hazard during transport and testing. A buffer with the ability to inactivate SARS-CoV-2 at the time of sample collection could simplify and expand testing for COVID-19 to non-conventional settings.

methodsWe evaluated a guanidium thiocyanate-based buffer, eNAT™ (Copan) as a possible transport and inactivation medium for downstream RT-PCR testing to detect SARS-CoV-2. Inactivation of SARS-CoV-2 USA-WA1/2020 in eNAT and in diluted saliva was studied at different incubation times. The stability of viral RNA in eNAT was also evaluated for up to 7 days at room temperature (28°C), refrigerated conditions (4°C) and at 35°C.

resultsSARS-COV-2 virus spiked directly in eNAT could be inactivated at >5.6 log

conclusioneNAT and similar guanidinium thiocyanate-based media may be of value for transport, preservation, and processing of clinical samples for RT-PCR based SARS-CoV-2 detection.

Identifiers

PMID33501462
PMCPMC7836134
OpenAlexW3125765293

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.