Evidence map›Paper›PMID 33501433›Full record

ArticlebioRxiv : the preprint server for biology2021

Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine.

Yasunori Watanabe, Luiza Mendonça, Elizabeth R Allen, Andrew Howe, Mercede Lee, Joel D Allen, Himanshi Chawla, David Pulido, Francesca Donnellan, Hannah Davies and 13 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 30 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors at 5 institutions in 2 countries.

Yasunori WatanabeSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Luiza MendonçaDivision of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK.
Elizabeth R AllenThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Andrew HoweElectron Bio-imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.
Mercede LeeThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Joel D AllenSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Himanshi ChawlaSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
David PulidoThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Francesca DonnellanThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Hannah DaviesThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Marta UlaszewskaThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Sandra Belij-RammerstorferThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Susan MorrisThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Anna-Sophia KrebsDivision of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK.
Wanwisa DejnirattisaiThe Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
Juthathip MongkolsapayaThe Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
Piyada SupasaThe Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
Gavin R ScreatonThe Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
Catherine M GreenThe Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
Teresa LambeThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Peijun ZhangDivision of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK.
Sarah C GilbertThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Max CrispinSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
University of Oxford · GBCentre for Human Genetics · GBUniversity of Southampton · GBDiamond Light Source · GBSiriraj Hospital · TH

Funding

Identification of neutralizing epitopes on SARS-CoV-2 spike for design of vaccines and small-molecule antiviralsUM1AI144462 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI BURTON, DENNIS R. · 2019 to 2025
$201.6M
Technology Development 2: MAS NMR and dynamic nuclear polarization for HIV-1 structural biologyP50AI150481 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRONENBORN, ANGELA M. · 2019 to 2021
$14.1M
NIAID NIH HHS P50 AI150481NIAID NIH HHS UM1 AI144462Wellcome Trust
6 · The paper itself

Abstract

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirms the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

Identifiers

PMID33501433
PMCPMC7836103
OpenAlexW3122919885

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.