Evidence map›Paper›PMID 33499884›Full record

ArticleCancer cell international2021

Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer.

W Wang, J Qiu, P Qu, H Chen, J Lan, H Chen, L Li, M Gu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

W WangDepartment of Urology, Jiangsu Provincial People's Hospital, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, China.
J QiuDepartment of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
P QuDepartment of Urology, Yancheng First People's Hospital, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224000, Jiangsu, China.
H ChenDepartment of Haematology, Yancheng First People's Hospital, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224000, Jiangsu, China.
J LanDepartment of Pathology, Yancheng First People's Hospital, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224000, Jiangsu, China.
H ChenDepartment of Pathology, Yancheng First People's Hospital, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224000, Jiangsu, China.
L LiTranslational Medicine Center, Yancheng First People's Hospital, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224000, Jiangsu, China.
M GuDepartment of Urology, Jiangsu Provincial People's Hospital, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, China. lancetgu@aliyun.com.ORCID http://orcid.org/0000-0002-9110-8054
Xuzhou Medical College · CNNanjing Medical University · CNShanghai Jiao Tong University · CN

Funding

Key Young Medical Talents in Jiangsu Province, China QNRC2016472Natural Science Foundation of Jiangsu Province BK20181212
6 · The paper itself

Abstract

backgroundThe regulator of cullins-1 (ROC1) is an essential subunit in the cullin-RING ligase (CRL) protein complex and has been shown to be critical in bladder cancer cell survival and progression. This study aimed to explore the molecular mechanism of ROC1 action in the malignant progression of bladder cancer.

methodsThis study utilized ex vivo, in vitro, and in vivo nude mouse experiments to assess the underlying mechanisms of ROC1 in bladder cancer cells. The expression of the components of the sonic hedgehog (SHH) pathway was determined by western blot analysis. ROC1 expression in human tumors was evaluated by immunohistochemistry.

resultsROC1 overexpression promoted the growth of bladder cancer cells, whereas knockdown of ROC1 expression had the opposite effect in bladder cancer cells. Mechanistically, ROC1 was able to target suppressor of fused homolog (SUFU) for ubiquitin-dependent degradation, allowing Gli2 release from the SUFU complex to activate the SHH pathway. Furthermore, knockdown of SUFU expression partially rescued the ROC1 knockdown-suppressed SHH activity as well as cancer cell growth inhibition. In ex vivo experiments, tissue microarray analysis of human bladder cancer specimens revealed a positive association of ROC1 expression with the SHH pathway activity.

conclusionThis study demonstrated that dysregulation of the ROC1-SUFU-GLI2 axis plays an important role in bladder cancer progression and that targeting ROC1 expression is warranted in further investigations as a novel strategy for the future control of bladder cancer.

Indexed as

Bladder cancerGli2ROC1Sonic hedgehog signalingSUFU

Identifiers

PMID33499884
PMCPMC7836478
OpenAlexW3116796191

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.