Evidence map›Paper›PMID 33499800›Full record

ArticleBMC neuroscience2021

Docosahexaenoic fatty acid reduces the pro-inflammatory response induced by IL-1β in astrocytes through inhibition of NF-κB and AP-1 transcription factor activation.

Emilia Zgórzyńska, Dawid Stulczewski, Barbara Dziedzic, Kuan-Pin Su, Anna Walczewska

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Emilia ZgórzyńskaDepartment of Cell-to-Cell Communication, Medical University of Lodz, Mazowiecka 6/8, 92- 215, Lodz, Poland. emilia.zgorzynska@umed.lodz.pl.ORCID 0000-0002-5411-3778
Dawid StulczewskiDepartment of Cell-to-Cell Communication, Medical University of Lodz, Mazowiecka 6/8, 92- 215, Lodz, Poland.
Barbara DziedzicDepartment of Cell-to-Cell Communication, Medical University of Lodz, Mazowiecka 6/8, 92- 215, Lodz, Poland.
Kuan-Pin SuAn-Nan Hospital, China Medical University, Tainan, Taiwan.
Anna WalczewskaDepartment of Cell-to-Cell Communication, Medical University of Lodz, Mazowiecka 6/8, 92- 215, Lodz, Poland.
Medical University of Lodz · PLChina Medical University · TW

Funding

China Medical University Hospital, Taichung, Taiwan CRS-108-048China Medical University Hospital, Taichung, Taiwan DMR-108-216China Medical University Hospital, Taichung, Taiwan DMR-109-102Ministry of Science and Technology, Taiwan 108-2314-B-039-016Ministry of Science and Technology, Taiwan 108-2813-C-039-133-BNarodowe Centrum Badań i Rozwoju DKO/PL-TW1/4/2013Uniwersytet Medyczny w Lodzi 502-03/0-079-04/502-04-035Uniwersytet Medyczny w Lodzi 503/0-079-04/503-01-001
6 · The paper itself

Abstract

backgroundAstrocytes are responsible for a broad range of functions that maintain homeostasis in the brain. However, their response to the pro-inflammatory cytokines released by activated microglia in various neurological pathologies may exacerbate neurodegenerative processes. Accumulating evidence suggests that omega-3 docosahexaenoic fatty acid (DHA) has an anti-inflammatory effect in various cell cultures studies and in a variety of neurological disorders. In this study we examined the mechanism involved in the inhibition of the pro-inflammatory response by DHA in astrocytes treated with IL-1β. METHODS AND

resultsActivation of the transcription factors NF-κB and AP-1 was measured in IL-1β-treated primary astrocytes incubated with various concentrations of DHA. COX-2 and iNOS protein expression was determined by Western blot, and TNF-α and IL-6 secretion was measured using ELISA-based assays. DHA treatment inhibited translocation of p65NF-κB to the nucleus, significantly lowered p65NF-κB protein level and fluorescence of p65NF-κB in the nucleus, reduced dose-dependently IκB protein phosphorylation, and the binding of the AP-1 transcription factor members (c-Jun/c-Fos) to the specific TPA-response element (TRE) of DNA. In addition, the expression of pro-inflammatory COX-2 and iNOS proteins was downregulated and TNF-α and IL-6 secretion was also reduced.

conclusionsThese results indicate that DHA is a powerful factor that reduces the pro-inflammatory response in astrocytes. Consequently, successful introduction of DHA into the astrocyte membranes can attenuate neuroinflammation, which is a key factor of age-related neurodegenerative disorders.

Indexed as

AnimalsAnti-Inflammatory AgentsAstrocytesDocosahexaenoic AcidsInflammationInterleukin-1betaNF-kappa BRatsTranscription Factor AP-1Anti-Inflammatory AgentsDocosahexaenoic AcidsInterleukin-1betaNF-kappa BTranscription Factor AP-1AstrocytesDocosahexaenoic acidNeuroinflammation

Identifiers

PMID33499800
PMCPMC7839194
OpenAlexW3122673963

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.