ArticleBMC microbiology2021
Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06095076 (Prospective Descriptive Study of the Cutaneous Microbiota of ICU Patients With Central Venous Catheter), which is not on this map. Cited by 199 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Prospective Descriptive Study of the Cutaneous Microbiota of ICU Patients With Central Venous Catheter (ICMc)
Who cites it
199 citing papers in PubMed, 1 synthesis or guideline pooled it, 336 citations in OpenAlex.
- Meta-analysis of caries microbiome studies can improve upon disease prediction outcomes.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Pooled it
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- Integrated morpho-biochemical, molecular, phylogenetic, and thermodynamic characterization of Bacillus isolates from ecologically distinct Indian soils.Molecular biology reports · 2026Article
- The lung microbiome in childhood-onset severe neuromuscular disease with respiratory insufficiency: rationale, current evidence, and opportunities for oxford nanopore long-read sequencing.Molecular and cellular pediatrics · 2026Review
- Bacterial metagenomic analysis of patients with chronic kidney disease undergoing hemodialysis based on 16S rDNA amplicon sequencing.Biomedical reports · 2026Article
- A scalable and cost-effective nanopore workflow for 16S rRNA microbiome profiling validated in a zebrafish gut model.Microbial genomics · 2026Article
- A comparative analysis of gut microbiome in dogs using short- and long-reads of 16S rRNA sequences reveals workflow-dependent biases.Veterinary research communications · 2026Article
- The Establishment of an Indigenous-Led Drinking Water Monitoring Program Leveraging qPCR and Metagenomics Testing in New Zealand.Water environment research : a research publication of the Water Environment Federation · 2026Article
- Longitudinal analysis of broiler cecal microbiome: unraveling the species level dynamics across age and sex.Poultry science · 2026Article
- Integrating host-microbiome multi-omics with machine learning: methods, benchmarks, and translational applications.Science China. Life sciences · 2026Review
- Comparative analysis of MinION and MiSeq using 16S rRNA gene amplicon sequencing in human gut microbiome.BMC biotechnology · 2026Article
- Quantitative evaluation of microbiome sequencing resolution under varying experimental conditions using defined mock communities.Scientific reports · 2026Article
- Distinct early gut microbiota patterns by delivery mode within 100 hours of birth.World journal of clinical pediatrics · 2026Article
- Canine Fecal Microbiome Dataset: Ultra-deep Multi-platform Sequencing Across Extraction and Library Protocols.Scientific data · 2026Article
- Full-Length 16S rRNA Amplicon Sequencing for the Simple and Simultaneous Detection of Multiple Probiotic Species in Commercial Products.Journal of microbiology and biotechnology · 2026Article
- Gut Microbiota and Chronic Kidney Disease: A Complex Interplay with Implications for Diagnosis and Treatment.Probiotics and antimicrobial proteins · 2026Review
- Probiotic-Induced Gut Microbiota Modulation: A Comparative Analysis Using 16S rRNA V3-V4 and Targeted Sequencing.Microorganisms · 2026Article
- Gut microbiome features associated with Bifidobacterium colonization predict personalized probiotic persistence patterns.Nature communications · 2026Article
- Oral microbiome profiling of primary oral candidiasis during infection and post-antifungal therapy.Clinical oral investigations · 2026Review
- Life-Stage-Dependent Variation in Gastrointestinal Microbial Communities of Drosophila melanogaster.Microbial ecology · 2026Article
139 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 7 institutions in 1 country.
Funding
Abstract
backgroundSpecies-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and treatment. Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the taxonomic classification of bacteria. This study aims to improve the method for full-length 16S rRNA gene analysis using the nanopore long-read sequencer MinION™. We compared it to the conventional short-read sequencing method in both a mock bacterial community and human fecal samples.
resultsWe modified our existing protocol for full-length 16S rRNA gene amplicon sequencing by MinION™. A new strategy for library construction with an optimized primer set overcame PCR-associated bias and enabled taxonomic classification across a broad range of bacterial species. We compared the performance of full-length and short-read 16S rRNA gene amplicon sequencing for the characterization of human gut microbiota with a complex bacterial composition. The relative abundance of dominant bacterial genera was highly similar between full-length and short-read sequencing. At the species level, MinION™ long-read sequencing had better resolution for discriminating between members of particular taxa such as Bifidobacterium, allowing an accurate representation of the sample bacterial composition.
conclusionsOur present microbiome study, comparing the discriminatory power of full-length and short-read sequencing, clearly illustrated the analytical advantage of sequencing the full-length 16S rRNA gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.