ArticleAnimals : an open access journal from MDPI2021
Multiparametric Study of Antioxidant Effect on Ram Sperm Cryopreservation-From Field Trials to Research Bench.
Article in Animals : an open access journal from MDPI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Effects of Supplementing Semen Extenders With Spirulina platensis Extract on Post-Thaw Ram Sperm Quality.Veterinary medicine and science · 2026Article
- Dietary Antioxidants and Nrf2-Related Redox Responses in Sheep: Evidence, Limitations, and Implications for Health and Productivity.Veterinary sciences · 2026Review
- Liquid Storage and Cryopreservation of Ram Semen: Storage-Associated Damage and the Role of Non-Enzymatic Antioxidants.Veterinary sciences · 2026Review
- Lyophilized Coffee Grounds as a Natural Antioxidant to Preserve Post-Thaw Sperm Quality in Dorper and Creole Rams.Animals : an open access journal from MDPI · 2026Article
- Article
- Dose-Dependent Effects of Protocatechuic Acid on Motility, Redox Balance, and DNA Integrity of Frozen-Thawed Ram Spermatozoa.International journal of molecular sciences · 2026Article
- Effects of Green Tea Extract and (+)-Catechin Hydrate on Sperm Quality and Seminal Plasma Antioxidant Activity: An In Vitro Study in Rams.Veterinary medicine and science · 2026Article
- Regularized canonical correlation models improve prediction of weather impacts on semen quality in Murciano-Granadina bucks.Veterinary and animal science · 2026Article
- Chitosan-coated nano-green-tea (Nano-GTE) in a tris-soybean lecithin extender improves post-thaw motility, antioxidant status, DNA integrity, and fertility of Ossimi Ram Semen.Scientific reports · 2026Article
- Mitochondrial specific antioxidant MitoPBN mitigates oxidative stress and improves mitochondrial function in cryopreserved ram sperm.Scientific reports · 2025Article
- Advancements in Understanding and Enhancing Antioxidant-Mediated Sperm Cryopreservation in Small Ruminants: Challenges and Perspectives.Antioxidants (Basel, Switzerland) · 2024Review
- The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment.Frontiers in veterinary science · 2024Article
- Article
- Impact of the Antioxidant Hydroxytyrosol on the Quality of Post-Thawed Stallion Semen.Veterinary medicine international · 2024Article
- Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender.Animals : an open access journal from MDPI · 2023Article
- Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing.Veterinary sciences · 2023Article
- Enhancing post-thaw quality of ram epididymal sperm by supplementation of rutin in cryopreservation extender.Scientific reports · 2023Article
- Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen.F1000Research · 2023Article
- Animal Reproduction: Semen Quality Assessment.Animals : an open access journal from MDPI · 2022Article
- Frequency of Semen Collection Affects Ram Sperm Cryoresistance.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The optimization of sperm cryopreservation protocols in ram is a feasible tool to reinforce artificial insemination technologies considering the desirable application of sperm by vaginal/cervical or transcervical deposition. Cryopreservation provokes different types of damage on spermatozoa and many of these detrimental effects are triggered by redox deregulation. For this reason, the antioxidant supplementation in sperm cryopreservation protocols to decrease reactive oxygen species (ROS) levels and to equilibrate redox status has been widely employed in different species. Despite this, more fertility trials are necessary to provide the definitive tool to ensure the antioxidant effectiveness on sperm quality. For this reason, in this work, we performed a multiparametric analysis of some previously tested antioxidants (crocin, GSH and Trolox) on ram sperm cryopreservation from field trials to sperm quality analyses focused on new strategies to measure redox balance. Attending to fertility trial, Trolox supplementation registered an improvement concerning to fertility (when we considered high fertility males) and multiple lambing frequency and other complementary and descriptive data related to lambing performance such as prolificacy and fecundity. This positive effect was more evident in multiple lambing frequency when we considered low fertility males than in global male analysis. In vitro analyses of sperm quality confirmed in vivo trials registering a positive effect on sperm viability and redox balance. In this study, we provided the definitive evidence that the role of trolox on redox balance maintenance has a direct effect on fertility parameters, such as prolificacy. The effectiveness of antioxidant treatments was tested, for the first time in ovine species, using an integrative and multiparametric approach combining in vivo and in vitro analyses and novel approaches, such as RedoxSYS. These types of strategies should be applied to improve sperm conservation methods and optimize AI technologies upgrading the correlation between in vitro and in vivo analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.