Evidence map›Paper›PMID 33498275›Full record

ArticlePlants (Basel, Switzerland)2021

A ClearSee-Based Clearing Protocol for 3D Visualization of

Ayame Imoto, Mizuki Yamada, Takumi Sakamoto, Airi Okuyama, Takashi Ishida, Shinichiro Sawa, Mitsuhiro Aida

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Expression of the auxin biosynthetic genesPlant biotechnology (Tokyo, Japan) · 2022
    Article
  10. Article
  11. Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution.International journal of molecular sciences · 2021
    Article
  12. Article
  13. The boundary-expressedPlant biotechnology (Tokyo, Japan) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ayame ImotoGraduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Nara 630-0192, Japan.
Mizuki YamadaInternational Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Takumi SakamotoFaculty of Science, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Airi OkuyamaFaculty of Science, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Takashi IshidaInternational Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.ORCID 0000-0001-5167-9971
Shinichiro SawaGraduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.ORCID 0000-0002-9309-9104
Mitsuhiro AidaInternational Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.ORCID 0000-0001-9000-7502

Funding

Japan Society for the Promotion of Science 16K07401Ministry of Education, Culture, Sports, Science and Technology 18H04842Ministry of Education, Culture, Sports, Science and Technology 20H04889Ministry of Education, Culture, Sports, Science and Technology 24114009Takeda Science Foundation 2009
6 · The paper itself

Abstract

Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable clearing method that is applicable to a wide range of tissues and is suitable for gene expression analysis using fluorescent reporters, but its application to the

Indexed as

3D imagingArabidopsis thalianacell wall stainingclearingconfocal microscopyembryofluorescent reporterGFP

Identifiers

PMID33498275
PMCPMC7909245

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.