Evidence map›Paper›PMID 33497534›Full record

ArticleCurrent protocols2021

Laboratory Maintenance and Culture of Pseudogymnoascus destructans, the Fungus That Causes Bat White-Nose Syndrome.

David S Blehert, Jeffrey M Lorch

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

David S BlehertU.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin.
Jeffrey M LorchU.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pseudogymnoascus destructans is a fungal pathogen that causes white-nose syndrome, an emerging and fatal disease of North American bats that has led to unprecedented population declines. As a psychrophile, P. destructans is adapted to infect bats during winter hibernation, when host metabolic activity and core body temperature are greatly reduced. The ability to maintain and cultivate isolates of P. destructans in the laboratory is necessary for conducting research with this fungus. This article describes protocols for culturing P. destructans from bat wing skin and soil, for cryopreserving the fungus, and for preparing liquid suspensions for laboratory experimentation. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Isolating Pseudogymnoascus destructans from bat wing skin Basic Protocol 2: Isolating Pseudogymnoascus destructans from soil Basic Protocol 3: Cryopreservation of Pseudogymnoascus destructans Basic Protocol 4: Preparing liquid conidial suspension of Pseudogymnoascus destructans.

Indexed as

AscomycotaChiropteraHibernationAnimalsLaboratoriesbatfungal pathogenwhite-nose syndromewildlife disease

Identifiers

PMID33497534

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.