Evidence map›Paper›PMID 33497020›Full record

ArticleArthritis & rheumatology (Hoboken, N.J.)2021

Anti-Cytosolic 5'-Nucleotidase 1A Autoantibodies Are Absent in Juvenile Dermatomyositis.

Anke Rietveld, Judith Wienke, Eline Visser, Wilma Vree Egberts, Wolfgang Schlumberger, Baziel van Engelen, Annet van Royen-Kerkhof, Hui Lu, Lucy Wedderburn, Christiaan Saris and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis & rheumatology (Hoboken, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Anke RietveldDonders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, Nijmegen, The Netherlands.ORCID 0000-0002-0242-4275
Judith WienkeUniversity Medical Center Utrecht and Utrecht University, Utrecht, The Netherlands.ORCID 0000-0002-3835-6374
Eline VisserRadboud Institute for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Wilma Vree EgbertsRadboud Institute for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Wolfgang SchlumbergerInstitute for Experimental Immunology, Euroimmun, Lübeck, Germany.
Baziel van EngelenDonders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, Nijmegen, The Netherlands.
Annet van Royen-KerkhofWilhelmina Children's Hospital, University Medical Center Utrecht, and Utrecht University, Utrecht, The Netherlands.
Hui LuUniversity of Bath, Bath, UK.
Lucy WedderburnNIHR Great Ormond Street Hospital Biomedical Research Centre, Centre for Adolescent Rheumatology Versus Arthritis, University College London Great Ormond Street Institute of Child Health, London, UK.ORCID 0000-0002-7495-1429
Christiaan SarisDonders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, Nijmegen, The Netherlands.
Sarah TansleyUniversity of Bath, Bath, UK.
Ger PruijnRadboud Institute for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.ORCID 0000-0001-7208-6200
Juvenile Dermatomyositis Research Group and the Dutch Myositis Consortium
Radboud University Nijmegen · NLUniversity of Bath · GBUtrecht University · NLEuroimmun Medizinische Labordiagnostika (Germany) · DEGreat Ormond Street Hospital · GB

Funding

Medical Research Council MR/N003322/1Versus Arthritis 21552
6 · The paper itself

Abstract

objectiveTo assess anti-cytosolic 5'-nucleotidase 1A (anti-cN-1A) autoantibodies in children with juvenile dermatomyositis (DM) and healthy controls, using 3 different methods of antibody detection, as well as verification of the results in an independent cohort.

methodsAnti-cN-1A reactivity was assessed in 34 Dutch juvenile DM patients and 20 healthy juvenile controls using the following methods: a commercially available full-length cN-1A enzyme-linked immunosorbent assay (ELISA), a synthetic peptide ELISA, and immunoblotting with a lysate from cN-1A-expressing HEK 293 cells. Sera from juvenile DM patients with active disease and those with disease in remission were analyzed. An independent British cohort of 110 juvenile DM patients and 43 healthy juvenile controls was assessed using an in-house full-length cN-1A ELISA.

resultsAnti-cN-1A reactivity was not present in sera from juvenile DM patients or healthy controls when tested with the commercially available full-length cN-1A ELISA or by immunoblotting, in either active disease or disease in remission. Additionally, in the British juvenile DM cohort, anti-cN-1A reactivity was not detected. Three Dutch juvenile DM patients had weakly positive results for 1 of 3 synthetic cN-1A peptides measured by ELISA.

conclusionJuvenile DM patients and young healthy individuals did not show anti-cN-1A reactivity as assessed by different antibody detection techniques.

Indexed as

5'-NucleotidaseAdolescentAutoantibodiesCase-Control StudiesChildChild, PreschoolDermatomyositisEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoblottingMale5'-NucleotidaseAutoantibodiesNT5C1A protein, human

Identifiers

PMID33497020
PMCPMC8360054
OpenAlexW3122462894

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.