ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2021
A streamlined whole blood CyTOF workflow defines a circulating immune cell signature of COVID-19.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 3 of them syntheses that pooled it.
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Who cites it
35 citing papers in PubMed, 3 syntheses or guidelines pooled it, 46 citations in OpenAlex.
- Individuals with JAK1 variants are affected by syndromic features encompassing autoimmunity, atopy, colitis, and dermatitis.The Journal of experimental medicine · 2024Pooled it
- Automated and reproducible cell identification in mass cytometry using neural networks.Briefings in bioinformatics · 2023Pooled it
- CD56-negative NK cells: Frequency in peripheral blood, expansion during HIV-1 infection, functional capacity, and KIR expression.Frontiers in immunology · 2022Pooled it
- Monocytes in pneumonia: Functional plasticity and innate memory (Review).International journal of molecular medicine · 2026Review
- Effects of refined carbohydrates on immune markers after weight loss on a very-low-carbohydrate diet: A randomized controlled feeding trial.Cell press blue · 2026Article
- Deep learning representations of human Immune Health for precision immunology.bioRxiv : the preprint server for biology · 2026Article
- Synovial Fluid Cell Profiling Identifies Distinct Inflammatory Phenotypes in Late-Onset Rheumatoid Arthritis and Polymyalgia Rheumatica.ACR open rheumatology · 2026Article
- Review
- Perturbation of the preterm human immune system in early life.JCI insight · 2026Article
- Cytokine-Driven Janus Kinase Signal Transducer and Activator of Transcription Pathway Hyperactivity Predicts Disease Severity in Pediatric FSGS.Kidney360 · 2026Article
- Perturbation of the Preterm Human Immune System in Early Life.medRxiv : the preprint server for health sciences · 2025Article
- Automated cytometric gating with human-level performance using bivariate segmentation.Nature communications · 2025Article
- Increased interferon I signaling, DNA damage response and evidence of T-cell exhaustion in a patient with combined interferonopathy (Aicardi-Goutières Syndrome, AGS) and cohesinopathy (Cornelia de Lange Syndrome, CdLS).Pediatric rheumatology online journal · 2025Article
- Immunophenotyping of Patients With Rheumatoid Arthritis Reveals Difference in CD27Mediators of inflammation · 2025Article
- Integration of T cell repertoire, CyTOF, genotyping and symptomatology data reveals subphenotypic variability in COVID-19 patients.Computational and structural biotechnology journal · 2025Article
- Barcoding of viable peripheral blood mononuclear cells with selenium and tellurium isotopes for mass cytometry experiments.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2024Article
- Cytometry masked autoencoder: An accurate and interpretable automated immunophenotyper.Cell reports. Medicine · 2024Article
- Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro.Communications biology · 2024Article
- Automated Cytometric Gating with Human-Level Performance Using Bivariate Segmentation.bioRxiv : the preprint server for biology · 2024Article
- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.Methods in molecular biology (Clifton, N.J.) · 2024Review
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Authors and funding
16 authors at 1 institution in 1 country.
Funding
Abstract
Mass cytometry (CyTOF) represents one of the most powerful tools in immune phenotyping, allowing high throughput quantification of over 40 parameters at single-cell resolution. However, wide deployment of CyTOF-based immune phenotyping studies are limited by complex experimental workflows and the need for specialized CyTOF equipment and technical expertise. Furthermore, differences in cell isolation and enrichment protocols, antibody reagent preparation, sample staining, and data acquisition protocols can all introduce technical variation that can confound integrative analyses of large data-sets of samples processed across multiple labs. Here, we present a streamlined whole blood CyTOF workflow which addresses many of these sources of experimental variation and facilitates wider adoption of CyTOF immune monitoring across sites with limited technical expertise or sample-processing resources or equipment. Our workflow utilizes commercially available reagents including the Fluidigm MaxPar Direct Immune Profiling Assay (MDIPA), a dry tube 30-marker immunophenotyping panel, and SmartTube Proteomic Stabilizer, which allows for simple and reliable fixation and cryopreservation of whole blood samples. We validate a workflow that allows for streamlined staining of whole blood samples with minimal processing requirements or expertise at the site of sample collection, followed by shipment to a central CyTOF core facility for batched downstream processing and data acquisition. We apply this workflow to characterize 184 whole blood samples collected longitudinally from a cohort of 72 hospitalized COVID-19 patients and healthy controls, highlighting dynamic disease-associated changes in circulating immune cell frequency and phenotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.