ArticleScientific reports2021
The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses.Plant biotechnology journal · 2026Article
- Direct and indirect responses of the Arabidopsis transcriptome to an induced increase in trehalose 6-phosphate.Plant physiology · 2024Article
- Synthetic developmental biology: molecular tools to re-design plant shoots and roots.Journal of experimental botany · 2023Review
- In vivo protein kinase activity of SnRK1 fluctuates in Arabidopsis rosettes during light-dark cycles.Plant physiology · 2023Article
- Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators.Plant biotechnology journal · 2022Article
- Rising rates of starch degradation during daytime and trehalose 6-phosphate optimize carbon availability.Plant physiology · 2022Article
- Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research.International journal of molecular sciences · 2022Review
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Abstract
The AlcR fungal protein responds to ethanol and binds to the fungal pAlcA promoter in its presence. This system was transferred to plants over twenty years ago and was claimed to function in the same manner in plants. However, never has the control experiment with plants containing the AlcR gene alone, with no downstream inducible construct, been made. In this paper, I conduct several experiments with this control, growing p35:AlcR plants in the presence or absence of ethanol. I found that when these plants were grown in the presence of ethanol, growth in several tissues and several stages of growth was retarded. This demonstrates that this system is not suitable for use in the plant sciences, and casts doubt on the conclusions of papers that have published phenotypes using this system.
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