ArticleNature communications2021
Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Protein-DNA Binding Sites Prediction via Integrating Pretrained Large Language Models and Contrastive Learning.Interdisciplinary sciences, computational life sciences · 2026Article
- The Fragmented Nature of Biosensor Development: Challenges and Paths to Mitigation.Biosensors · 2026Review
- A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Toxicity of formaldehyde, and its role in the formation of harmful and aromatic compounds during food processing.Food chemistry: X · 2025Review
- Identification and characterization of a novel formaldehyde dehydrogenase inApplied and environmental microbiology · 2024Article
- A pyridinium-based strategy for lysine-selective protein modification and chemoproteomic profiling in live cells.Chemical science · 2024Article
- Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde.Synthetic and systems biotechnology · 2023Article
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Epidural Pulsation Accelerates the Drainage of Brain Interstitial Fluid.Aging and disease · 2023Article
- [Epidural photobiomodulation accelerates the drainage of brain interstitial fluid and its mechanism].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2022Article
- Systematic Screening of Trigger Moieties for Designing Formaldehyde Fluorescent Probes and Application in Live Cell Imaging.Biosensors · 2022Article
- Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism.Nature microbiology · 2022Article
- Origin and Fate of Acrolein in Foods.Foods (Basel, Switzerland) · 2022Review
- Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms.International journal of molecular sciences · 2022Review
- Biomimetic mimicry of formaldehyde-induced DNA-protein crosslinks in the confined space of a metal-organic framework.Chemical science · 2022Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR.Nature communications · 2021Article
- Functional asymmetry and chemical reactivity of CsoR family persulfide sensors.Nucleic acids research · 2021Article
- The cellular function and molecular mechanism of formaldehyde in cardiovascular disease and heart development.Journal of cellular and molecular medicine · 2021Review
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formaldehyde (FA) has long been considered as a toxin and carcinogen due to its damaging effects to biological macromolecules, but its beneficial roles have been increasingly appreciated lately. Real-time monitoring of this reactive molecule in living systems is highly desired in order to decipher its physiological and/or pathological functions, but a genetically encoded FA sensor is currently lacking. We herein adopt a structure-based study of the underlying mechanism of the FA-responsive transcription factor HxlR from Bacillus subtilis, which shows that HxlR recognizes FA through an intra-helical cysteine-lysine crosslinking reaction at its N-terminal helix α1, leading to conformational change and transcriptional activation. By leveraging this FA-induced intra-helical crosslinking and gain-of-function reorganization, we develop the genetically encoded, reaction-based FA sensor-FAsor, allowing spatial-temporal visualization of FA in mammalian cells and mouse brain tissues.
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