ArticleMicrobial cell factories2021
Investigation of Kluyveromyces marxianus as a novel host for large-scale production of porcine parvovirus virus-like particles.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- The first report of porcine parvovirus 8 detection and genetic analysis in South Korea.Frontiers in veterinary science · 2026Article
- Porcine Parvovirus virus-like particle vaccine induces long-term humoral immunity by recruiting T follicular helper and germinal center B cell responses.Frontiers in immunology · 2026Article
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- Enhancing recombinant protein production through Cre-loxP mediated chromosomal rearrangement evolution in Kluyveromyces marxianus.Communications biology · 2025Article
- Characterization and optimization of mnn11Δ-mediated enhancement in heterologous protein production in Kluyveromyces marxianus.Microbial cell factories · 2025Article
- Alien Chromosome Serves as a Novel Platform for Multiple Gene Expression inMicroorganisms · 2025Article
- From genetic constraints to process hurdles: A review ofCurrent research in food science · 2025Review
- Synthesis and characterization of molecularly imprinted polymer nanoparticles against porcine circovirus type 2 viral-like particles.Analytical and bioanalytical chemistry · 2024Article
- High-Yield Expressed Human Ferritin Heavy-Chain Nanoparticles inFoods (Basel, Switzerland) · 2024Article
- Coupling thermotolerance and high production of recombinant protein by CYR1Communications biology · 2024Article
- Article
- Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery.Vaccines · 2023Review
- Development of modern immunization agent against bovine papillomavirus type 1 infection based on BPV1 L1 recombinant protein.Frontiers in veterinary science · 2023Article
- Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay.Viruses · 2022Article
- Establishment of a Cre-loxP System Based on a LeakyMicroorganisms · 2022Article
- Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature.MicrobiologyOpen · 2022Article
- BioprospectingFrontiers in bioengineering and biotechnology · 2022Review
- A Single Vaccination of IBDV Subviral Particles Generated byVaccines · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPorcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss and death and mummification in affected fetal pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions and highly immunostimulatory that can induce strong humoral immune and T cell responses with no risk of pathogenicity. The production of PPV VLPs is still a challenge based on traditional expression platforms due to their low yields and high culture costs. Kluyveromyces marxianus is a safe and fast-growing eukaryote that can get high biomass with low-cost cultures. In this study, we investigated the expression and downstream processes of PPV VLPs in K. marxianus, and the potential for effective stand-alone vaccines.
resultsAfter optimization according to the codon bias of K. marxianus, the VP2 protein from Kresse strain was highly expressed. In a 5 L fermentator, the yield of PPV VLPs reached 2.5 g/L, quantified by HPLC, using a defined mineral medium after 48 h fermentation. Two strategies were established to purify intracellular PPV VLPs: (i) Using the cation exchange chromatography coupled with Sephacryl® S-500 HR chromatography to purify VLPs from the supernatants of pH adjusted cell lysates. (ii) Using anion exchange chromatography followed by cross-flow diafiltration to recover the VLPs precipitated in pH adjusted cell lysates. The purity of PPV VLPs reached about 95%, and total recovery was more than 60%. Vaccination of mice with the purified PPV VLPs induced high titers of specific IgG antibodies in sera, and showed hemagglutination inhibitions on both swine and guinea pig erythrocytes. Spleen lymphocyte proliferation and cytokines detection suggested the PPV VLPs produced by K. marxianus provoked the cellular immune and humoral immunity responses in mice.
conclusionsThis is the highest production of recombinant PPV VLPs achieved to date. The superiorities, Generally Recognized As Safe (GRAS), high production, short lead time, and low cost, make K. marxianus a greatly competitive platform for bioproduction of PPV VLPs vaccine.
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