ArticleConnective tissue research2022
Regulation of chondrocyte biosynthetic activity by dynamic hydrostatic pressure: the role of TRP channels.
Article in Connective tissue research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Integrating Mechanical Loading, Mechanotransduction, and Biological Responses in Musculoskeletal Tissues Across the Lifespan: Regulation Influenced by Cells, Extracellular Matrix, and Sex.Results and problems in cell differentiation · 2026Review
- Role of transient receptor potential (TRP) channels in osteoarthritis: a comprehensive review.Frontiers in pharmacology · 2025Review
- Effect of load reduction on the calcification of rat tibial tuberosity: Focus on calcification factors and chondrocyte mechanosensors.Journal of anatomy · 2025Article
- Ion channels in osteoarthritis: emerging roles and potential targets.Nature reviews. Rheumatology · 2024Review
- Promoting Articular Cartilage Regeneration through Microenvironmental Regulation.Journal of immunology research · 2024Review
- NaNature · 2024Article
- Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.iScience · 2023Article
- [Research progress of chondrocyte mechanotransduction mediated by TRPV4 and PIEZOs].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Review
- The role of TRPV4 in the regulation of retinal ganglion cells apoptosis in rat and mouse.Heliyon · 2023Article
- Three-dimensional visualization of neural networks inside bone by Osteo-DISCO protocol and alteration of bone remodeling by surgical nerve ablation.Scientific reports · 2023Article
- Novel Insights into the Role of Keratinocytes-Expressed TRPV3 in the Skin.Biomolecules · 2023Review
- Mimicking Loading-Induced Cartilage Self-HeatingACS biomaterials science & engineering · 2023Article
- Mechanoregulatory role of TRPV4 in prenatal skeletal development.Science advances · 2023Article
- Network-based modelling of mechano-inflammatory chondrocyte regulation in early osteoarthritis.Frontiers in bioengineering and biotechnology · 2023Article
- PIEZO1 is downregulated in glenohumeral chondrocytes in early cuff tear arthropathy following a massive rotator cuff tear in a mouse model.Frontiers in bioengineering and biotechnology · 2023Article
- Local mechanical stimuli correlate with tissue growth in axolotl salamander joint morphogenesis.Proceedings. Biological sciences · 2022Article
- Mechanogenetics: harnessing mechanobiology for cellular engineering.Current opinion in biotechnology · 2022Review
- The Role of Mechanically-Activated Ion Channels Piezo1, Piezo2, and TRPV4 in Chondrocyte Mechanotransduction and Mechano-Therapeutics for Osteoarthritis.Frontiers in cell and developmental biology · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
introductionChondrocytes perceive and respond to mechanical loading as signals that regulate their metabolism. Joint loading exposes chondrocytes to multiple modes of mechanical stress, including hydrostatic pressure; however, the mechanisms by which chondrocytes sense physiologically relevant levels of hydrostatic pressure are not well understood. We hypothesized that hydrostatic pressure is transduced to an intracellular signal through mechanosensitive membrane ion channels of chondrocytes. The goals of this study were to examine the effect of hydrostatic loading on the development of engineered cartilage tissue and the contribution of mechanosensitive ion channels on these hydrostatic loading effects.
methodsUsing a 3D model of porcine chondrocytes in agarose, we applied specific chemical inhibitors to determine the role of transient receptor potential (TRP) ion channels TRPV1, TRPV4, TRPC3, and TRPC1 in transducing hydrostatic pressure.
resultsHydrostatic loading caused a frequency and magnitude-dependent decrease in sulfated glycosaminoglycans (S-GAG), without changes in DNA content. Inhibiting TRPC3 and TRPV4 decreased S-GAG content; however, only the inhibition of TRPV1 partially attenuated the hydrostatic loading-induced reduction in S-GAG content.
conclusionsOur findings indicate that TRPV1 may serve as a transducer of hydrostatic pressure in chondrocytes, and provide further support for the role of TRPV4 in regulating chondrocyte anabolism, as well as initial evidence implicating TRPC3 in chondrogenesis. These findings add to our further understanding of the chondrocyte "channelome" and suggest that a range of ion channels mediate the transduction of different biophysical stimuli such as hydrostatic pressure, membrane stretch, or osmotic stress.
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