Evidence map›Paper›PMID 33493647›Full record

ArticleNeuroscience letters2021

Roles of the synaptic molecules Hevin and SPARC in mouse neuromuscular junction development and repair.

Vanessa L Brayman, Thomas Taetzsch, MacKenzie Miko, Shreyaska Dahal, W Christopher Risher, Gregorio Valdez

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 2 pooled it
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 2 syntheses or guidelines pooled it, 9 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Alpha-neurexins in health and disease.Frontiers in molecular neuroscience · 2025
    Review
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Vanessa L BraymanFralin Biomedical Research Institute, Virginia Tech Carilion, 2 Riverside Circle, Roanoke, VA, 24016, USA; Graduate Program in Translational Biology, Medicine, and Health, Virginia Tech, 1 Riverside Circle, Roanoke, VA, 24016, USA.
Thomas TaetzschDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, 70 Ship St, Providence, RI, 02903, USA.
MacKenzie MikoFralin Biomedical Research Institute, Virginia Tech Carilion, 2 Riverside Circle, Roanoke, VA, 24016, USA.
Shreyaska DahalFralin Biomedical Research Institute, Virginia Tech Carilion, 2 Riverside Circle, Roanoke, VA, 24016, USA.
W Christopher RisherDepartment of Biomedical Sciences, Joan C. Edwards School of Medicine at Marshall University, 1 John Marshall Drive, Huntington, WV, 25755, USA.
Gregorio ValdezDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, 70 Ship St, Providence, RI, 02903, USA; Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University, Providence, United States; Department of Neurology, Warren Alpert Medical School of Brown University, Providence, United States. Electronic address: gregorio_valdez@brown.edu.
Carilion Roanoke Memorial Hospital · USAllen Institute for Brain Science · USBrown University · USMarshall University · US

Funding

Targeting the fibroblast growth factor binding protein-1 to slow degeneration of neuromuscular junctionsR01AG055545 · NIA · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI VALDEZ, GREGORIO · 2017 to 2021
$2.2M
Analysis of Sensory Neurons to Understand and Identify Factors to Treat ALSR21NS106313 · NINDS · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI VALDEZ, GREGORIO · 2018 to 2019
$433k
Synaptic FGFs are required and sufficient to maintain and repair aged NMJsR56AG051501 · NIA · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI VALDEZ, GREGORIO · 2015 to 2015
$377k
NIA NIH HHS R01 AG055545NIA NIH HHS R56 AG051501NINDS NIH HHS R21 NS106313
6 · The paper itself

Abstract

Hevin and secreted protein acidic and rich in cysteine (SPARC) are highly homologous matricellular proteins that function in concert to guide the formation of brain synapses. Here, we investigated the role of these glycoproteins in neuromuscular junction (NMJ) maturation, stability, and repair following injury. Hevin and SPARC mRNA levels in developing (postnatal day 9), adult (postnatal days 90 and 120), and injured (fibular nerve crush) skeletal muscles were assessed with qPCR. Muscle fiber size was analyzed in developing (P9) mice lacking SPARC, Hevin, and both SPARC and Hevin. NMJ morphology was assessed in developing (P9), adult (P90) and injured (fibular nerve crush) mice lacking SPARC, Hevin, and both SPARC and Hevin skeletal muscle. Hevin and SPARC are expressed in skeletal muscles and are upregulated following nerve injury. Hevin

Indexed as

AnimalsCalcium-Binding ProteinsCells, CulturedExtracellular Matrix ProteinsMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMice, TransgenicMyoblastsNeuromuscular JunctionOsteonectinSynapsesCalcium-Binding ProteinsExtracellular Matrix ProteinsOsteonectinSparcl1 protein, mouseSPARC protein, mouseDevelopmentExtracellular matrixInjuryNeuromuscular junctionSkeletal muscleSynapse

Identifiers

PMID33493647
PMCPMC8418192
OpenAlexW3124535488

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.