ArticleCurrent protocols2021
Targeted Genetic Changes in Candida albicans Using Transient CRISPR-Cas9 Expression.
Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- The transcription factor Ahr1 governs the recruitment of neutrophils to the brain and clearance ofmBio · 2026Article
- EditingBiomolecules · 2026Review
- Dietary tannic acid promotes intestinal clearance of C. albicans by cross-linking hyphal chitosan.PLoS pathogens · 2025Article
- Roles of P-body factors inbioRxiv : the preprint server for biology · 2025Article
- Roles of P-body factors in Candida albicans filamentation and stress response.PLoS genetics · 2025Article
- Streptococcus mutans regulates ubiquitin modification of Candida albicans in the bacterial-fungal interaction.PLoS pathogens · 2025Article
- Systematic analysis of themBio · 2024Article
- Article
- CWHM-974 is a fluphenazine derivative with improved antifungal activity againstAntimicrobial agents and chemotherapy · 2023Article
- Genetic Screening ofFrontiers in microbiology · 2022Article
Corrections and comments
- Erratum issued
- Erratum issued
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Candida albicans is an opportunistic fungal pathogen responsible for significant disease and mortality. Absent complete mating and other convenient methods, dissection of its virulence factors relies on robust tools to delete, complement, and otherwise modify genes of interest in this diploid organism. Here we describe the design principles and use of CRISPR associated nuclease 9 (Cas9) and single-guide RNAs transiently expressed from PCR cassettes to modify genes of interest, generating homozygous mutants in a single transformation step. © 2021 Wiley Periodicals LLC. Basic Protocol 1: PCR amplification of CRISPR components Basic Protocol 2: Transformation of Candida albicans Basic Protocol 3: Selecting and genotyping transformants Alternate Protocol 1: Deletion with recyclable markers by CRISPR induced marker excision (CRIME) Alternate Protocol 2: Knock-in and combining multiple cassettes with overlapping homology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.