ReviewReproductive medicine and biology2021
Mitochondrial replacement by genome transfer in human oocytes: Efficacy, concerns, and legality.
Review in Reproductive medicine and biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Ovarian ageing: from biological constraint to emerging therapeutic frontier.Journal of assisted reproduction and genetics · 2026Review
- Current state of the art in the transmission of mitochondrial DNA pathogenic variants: reviewing preventive-therapeutic alternatives.Journal of assisted reproduction and genetics · 2026Review
- Molecular Bases of Myopathies and Their Impact on Clinical Practice: Advances and Future Perspectives.International journal of molecular sciences · 2026Review
- The role of metabolic reprogramming in oocyte development: research progress and prospects.Frontiers in reproductive health · 2026Review
- Mitochondrial dysfunction in oocytes: implications for fertility and ageing.Journal of ovarian research · 2025Review
- Abnormalities of Oocyte Maturation: Mechanisms and Implications.International journal of molecular sciences · 2024Review
- Human ovarian aging is characterized by oxidative damage and mitochondrial dysfunction.Human reproduction (Oxford, England) · 2023Article
- Clinical Approaches for Mitochondrial Diseases.Cells · 2023Review
- Article
- Mitochondria Have Made a Long Evolutionary Path from Ancient Bacteria Immigrants within Eukaryotic Cells to Essential Cellular Hosts and Key Players in Human Health and Disease.Current issues in molecular biology · 2023Review
- Genome transfer technique for bovine embryo production using the metaphase plate and polar body.Journal of assisted reproduction and genetics · 2023Article
- Closing the knowledge void between healthy gametes and healthy offspring.Journal of assisted reproduction and genetics · 2022Article
- Enucleated oocyte donation: first for infertility treatment, then for mitochondrial diseases.Journal of assisted reproduction and genetics · 2022Article
- Mitochondrial replacement by genome transfer in human oocytes: Efficacy, concerns, and legality.Reproductive medicine and biology · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPathogenic mitochondrial (mt)DNA mutations, which often cause life-threatening disorders, are maternally inherited via the cytoplasm of oocytes. Mitochondrial replacement therapy (MRT) is expected to prevent second-generation transmission of mtDNA mutations. However, MRT may affect the function of respiratory chain complexes comprised of both nuclear and mitochondrial proteins.
methodsBased on the literature and current regulatory guidelines (especially in Japan), we analyzed and reviewed the recent developments in human models of MRT. MAIN
findingsMRT does not compromise pre-implantation development or stem cell isolation. Mitochondrial function in stem cells after MRT is also normal. Although mtDNA carryover is usually less than 0.5%, even low levels of heteroplasmy can affect the stability of the mtDNA genotype, and directional or stochastic mtDNA drift occurs in a subset of stem cell lines (mtDNA genetic drift). MRT could prevent serious genetic disorders from being passed on to the offspring. However, it should be noted that this technique currently poses significant risks for use in embryos designed for implantation.
conclusionThe maternal genome is fundamentally compatible with different mitochondrial genotypes, and vertical inheritance is not required for normal mitochondrial function. Unresolved questions regarding mtDNA genetic drift can be addressed by basic research using MRT.
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