Evidence map›Paper›PMID 33487503›Full record

Trial reportClinical nutrition (Edinburgh, Scotland)2021

A collagen hydrolysate/milk protein-blend stimulates muscle anabolism equivalently to an isoenergetic milk protein-blend containing a greater quantity of essential amino acids in older men.

M S Brook, P Scaife, J J Bass, J Cegielski, S Watanabe, D J Wilkinson, K Smith, B E Phillips, P J Atherton

Open access · hybridAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Clinical nutrition (Edinburgh, Scotland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

M S BrookMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK; School of Life Sciences, University of Nottingham, Derby, UK.
P ScaifeMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
J J BassMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
J CegielskiMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
S WatanabeMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
D J WilkinsonMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
K SmithMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
B E PhillipsMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK.
P J AthertonMRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, Clinical, Metabolic and Molecular Physiology, School of Medicine, University of Nottingham, Derby, UK. Electronic address: philip.atherton@nottingham.ac.uk.
Versus Arthritis · GBUniversity of Nottingham · GB

Funding

Medical Research Council MR/P021220/1Medical Research Council MR/R502364/1
6 · The paper itself

Abstract

BACKGROUND &

aimsNutritional composition is key for skeletal muscle maintenance into older age. Yet the acute effects of collagen protein blended with other protein sources, in relation to skeletal muscle anabolism, are ill-defined. We investigated human muscle protein synthesis (MPS) responses to a 20 g blend of collagen protein hydrolysate + milk protein (CP+MP, 125 ml) oral nutritional supplement (ONS) vs. 20 g non-blended milk protein source (MP, 200 ml) ONS, in older adults.

methodsHealthy older men (N = 8, 71±1 y, BMI: 27±1 kg·m

resultsMP resulted in a greater increase in plasma leucine (MP mean: 152 ± 6 μM, CP+MP mean: 113 ± 4 μM (Feed P < 0.001) and EAA (MP mean: 917 ± 25 μM, CP+MP mean: 786 ± 15 μM (Feed P < 0.01) than CP+MP. CP + MP increased plasma glycine (peak 385 ± 57 μM (P < 0.05)), proline (peak 323 ± 29 μM (P < 0.01)) and non-essential amino acids (NEAA) (peak 1621 ± 107 μM (P < 0.01)) with MP showing no increase. Plasma insulin increased in both trials (CP+MP: 58 ± 10 mU/mL (P < 0.01), MP: 42 ± 6 mU/mL (P < 0.01), with peak insulin greater with CP+MP vs. MP (P < 0.01). MPS demonstrated equivalent increases in response to CP+MP and MP under both FED (MP: 0.039 ± 0.005%/h to 0.081 ± 0.014%/h (P < 0.05), CP+MP: 0.042 ± 0.004%/h to 0.085 ± 0.007%/h (P < 0.05)) and FED-EX (MP: 0.039 ± 0.005%/h to 0.093 ± 0.013%/h (P < 0.01), CP+MP: 0.042 ± 0.004%/h to 0.105 ± 0.015%/h, (P < 0.01)) conditions. FED muscle p-mTOR fold-change from baseline increased to a greater extent with CP+MP vs. MP (P < 0.05), whilst FED-EX muscle p-eEF2 fold-change from baseline decreased to a greater extent with CP+MP vs. MP (P < 0.05); otherwise anabolic signaling responses were indistinguishable.

conclusionFresubin®3.2 kcal DRINK, which contains a 20 g mixed blend of CP+MP, resulted in equivalent MPS responses to MP alone. Fresubin® 3.2 Kcal DRINK may provide a suitable alternative to MP for use in older adults and a convenient way to supplement calories and protein to improve patient adherence and mitigate muscle mass loss.

Indexed as

CollagenDietary SupplementsFood, FormulatedMilk ProteinsProtein HydrolysatesAgedAmino AcidsAmino Acids, EssentialCross-Over StudiesHumansInsulinMaleMuscle ProteinsMuscle, SkeletalSignal TransductionAmino AcidsAmino Acids, EssentialCollagenInsulinMilk ProteinsMuscle ProteinsProtein HydrolysatesAgeingCollagenEssential amino acidsExerciseMuscle protein synthesisOral nutritional supplement

Identifiers

PMID33487503
PMCPMC8251659
OpenAlexW3120156927

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.