Evidence map›Paper›PMID 33485335›Full record

ArticleBMC veterinary research2021

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis.

Yufang Kong, Huiyu Wang, Shaoqiang Wu, Jizhou Lv, Lin Mei, Huifang Zhou, Xiangmei Lin, Xueqing Han

Open access · goldAbstract read
In one paragraph

Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yufang Kong *Institute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China.
Huiyu Wang *Institute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China.
Shaoqiang WuInstitute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China.
Jizhou LvInstitute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China.
Lin MeiInstitute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China.
Huifang ZhouPeople's Hospital of Jiaxiang, Jiaxiang County, Jining City, 272400, Shandong Province, China.
Xiangmei LinInstitute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China. linxm@caiq.org.cn.
Xueqing HanInstitute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China. 1422355351@qq.com.ORCID http://orcid.org/0000-0003-3779-5786
Chinese Academy of Inspection and Quarantine · CN

Funding

National Key Reseach and Development Program of China 2017YFD0501803Research Program of Chinese Academy of Inspection and Quarantine 2018JK012
6 · The paper itself

Abstract

backgroundBrucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum.

resultsThe test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The critical threshold for determining negative or positive through the ratio of the fluorescent signal of the test line and the control line (H

conclusionIn our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

Indexed as

AnimalsAntibodies, BacterialAntibodies, MonoclonalBrucellaBrucellosisCattleChromatography, AffinityGoatsMicrospheresQuantum DotsReagent StripsSensitivity and SpecificitySheepAntibodies, BacterialAntibodies, MonoclonalReagent StripsBrucellosisImmunochromatographic strip testQuantum dots fluorescent microspheres

Identifiers

PMID33485335
PMCPMC7823175
OpenAlexW3119926933

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.